Volume 23, Number 3—March 2017
Research
Zika Virus RNA Replication and Persistence in Brain and Placental Tissue
Julu Bhatnagar
, Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif Zaki
, Demi B. Rabeneck, Roosecelis B. Martines, Sarah Reagan-Steiner, Yokabed Ermias, Lindsey B.C. Estetter, Tadaki Suzuki, Jana M. Ritter, M. Kelly Keating, Gillian Hale, Joy Gary, Atis Muehlenbachs, Amy J. Lambert, Robert Lanciotti, Titilope Oduyebo, Dana Meaney-Delman, Fernando Bolaños, Edgar Alberto Parra Saad, Wun-Ju Shieh, and Sherif Zaki
Table 3
Characteristics and laboratory findings for 13 microcephaly-associated case-patients*
| Case-patient no. | Maternal travel history or residence | Maternal symptom onset, gestation wk/trimester | Outcome | End of pregnancy, gestational age, wk/trimester | Results of Zika virus testing performed on FFPE tissues |
|
|---|---|---|---|---|---|---|
| RT-PCR | ISH† | |||||
| 54 | Brazil | 4/first | Infant with microcephaly died 6 h after birth | 38/third | Positive (brain); negative (placenta, spleen, kidney, lung, liver) | Positive (brain) |
| 66 | Colombia | 8/first | Infant with microcephaly died 2 d after birth | 26/third | Positive (brain, placenta); negative (liver) | Positive (brain) |
| 67 | Colombia | 8/first | Infant with microcephaly died shortly after birth | 27/third | Positive (brain, placenta); negative (liver) | Positive (brain) |
| 68 | Colombia | 10/first | Infant with microcephaly died shortly after birth | 27/third | Positive (brain, placenta); negative (liver) | Positive (brain), negative (placenta) |
| 55 | Brazil | NA/first | Infant with microcephaly died few min after birth | 29/third | Positive (brain); negative (placenta) | Positive (brain) |
| 53 | Brazil | NA/first | Infant with microcephaly died 20 h after birth | 36/third | Positive (brain); negative (placenta, spleen, kidney, heart) | Positive (brain) |
| 37 | Brazil | NA/first | Infant with microcephaly died 60 d after birth | 38/third | Positive (brain) | Positive (brain) |
| 65 | Colombia | NA/first | Infant with microcephaly died few minutes after birth | 28/third | Positive (brain, placenta); negative (liver) | Negative (brain, placenta) |
| 49 | Brazil, delivered in USA | 7/first | Infant with nonfatal microcephaly | 37/third | Positive (placenta) | Tissue NA |
| 83 | Cape Verde‡ | 7/first | Infant with nonfatal microcephaly | 36/third | Positive (placenta) | Negative (placenta) |
| 20 | Marshall Islands‡ | Unknown | Infant with nonfatal microcephaly | 31/third | Positive (placenta) | Tissue NA |
| 85 | Honduras‡ | 10/first | Infant with nonfatal microcephaly | 37/third | Positive (placenta, umbilical cord) | Negative (placenta) |
| 13 | Dominican Republic | 18/second | Infant with nonfatal microcephaly | 39/third | Negative (placenta, umbilical cord, membrane) | ND |
*FFPE, formalin-fixed, paraffin-embedded; ISH, in situ hybridization; NA, not available; ND, not done; RT-PCR, reverse transcription PCR.
†ISH results include staining by sense and antisense probes.
‡Travel history.
Page created: February 17, 2017
Page updated: February 17, 2017
Page reviewed: February 17, 2017
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