Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France
, Mathieu Tourdjman1
, Alexandre Leclercq1
, Estelle Hamelin, Edith Laurent, Nathalie Fredriksen, Dieter Van Cauteren, Hélène Bracq-Dieye, Pierre Thouvenot, Guillaume Vales, Nathalie Tessaud-Rita, Mylène M. Maury, Andreea Alexandru, Alexis Criscuolo, Emmanuel Quevillon, Marie-Pierre Donguy, Vincent Enouf, Henriette de Valk, Sylvain Brisse2
, and Marc Lecuit2
Author affiliations: Institut Pasteur, Paris, France (A. Moura, A. Leclercq, H. Bracq-Dieye, P. Thouvenot, G. Vales, N. Tessaud-Rita, M. Maury, A. Alexandru, A. Criscuolo, E. Quevillon, V. Enouf, S. Brisse, M. Lecuit); Institut National de la Santé et de la Recherche Médicale Unité 1117, Paris (A. Moura, M.M. Maury, M. Lecuit); Santé Publique France, Saint-Maurice, France (M. Tourdjman, E. Laurent, D. Van Cauteren, H. de Valk); Ministry of Agriculture, Agrifood, and Forestry, Paris (E. Hamelin, N. Fredriksen, M.-P. Donguy); Paris Descartes University, Paris (M. Lecuit); Necker-Enfants Malades University Hospital, Paris (M. Lecuit)
Figure 2. Comparison of pulsed-field gel electrophoresis (PFGE) and core genome multilocus sequence typing (cgMLST) for surveillance of Listeria monocytogenes, France. A) Number of total types and number of types triggering epidemiologic alerts. B) Number of human isolates per epidemiologic alert. C) Number of types within epidemiologic alerts with identified source. D) Time delay (days) between obtaining isolate and typing results. Horizontal lines in panels B and D indicate medians, and boxes indicate 25th and 75th percentiles. Error bars indicate maximum and minimum values.
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