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Volume 24, Number 1—January 2018
Research

Detection and Circulation of a Novel Rabbit Hemorrhagic Disease Virus in Australia

Jackie E. Mahar, Andrew J. Read, Xingnian Gu, Nadya Urakova1, Roslyn Mourant, Melissa Piper, Stéphanie Haboury, Edward C. Holmes, Tanja Strive, and Robyn N. HallComments to Author 
Author affiliations: Commonwealth Scientific and Industrial Research Organisation (CSIRO), Acton, Australian Capital Territory, Australia (J.E. Mahar, N. Urakova, R. Mourant, M. Piper, S. Haboury, T. Strive, R.N. Hall); The University of Sydney, Sydney, New South Wales, Australia (J.E. Mahar, E.C. Holmes); Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia (A.J. Read, X. Gu); Invasive Animals Cooperative Research Centre, Bruce, Australian Capital Territory, Australia (N. Urakova, T. Strive, R.N. Hall)

Main Article

Table 3

Cross-reactivity of different diagnostic tests used to identify novel RHDVa variant in rabbits, Australia*

Test
RHDV
RHDVa
RCV-A1
RHDV antigen capture ELISA
+++
–/+

RCV-A1 blocking ELISA


+++
RHDV competition ELISA
+++
++
–/+
RHDV IgA ELISA
+++
+++
++
RHDV IgM ELISA
+++
+++
++
VP60 rRT-PCR
+++
+++
+++
RHDVXa-2010 rRT-PCR

+++

Lagovirus RT-PCR +++ +++ +++

*All tests used the VP60 capsid protein coding region as target region. RCV, rabbit calicivirus; RHDV, rabbit hemorrhagic disease virus; rRT-PCR, real-time reverse transcription PCR; VP, viral protein; –, no cross-reactivity; –/+, minimal cross-reactivity; ++, moderate cross-reactivity; +++, marked cross-reactivity.

Main Article

1Current affiliation: University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA.

Page created: January 09, 2018
Page updated: January 09, 2018
Page reviewed: January 09, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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