Volume 24, Number 12—December 2018
Capnocytophaga canimorsus Capsular Serovar and Disease Severity, Helsinki Hospital District, Finland, 2000–2017
||PCR typing†||Western blot typing‡||Serovar
|H11, H16, H23, H37, H39, H42, H48, H52, H56, H60, H62, H70, H74, H75, H76, H78, H80||+||+||+||–||–||–||+||–||–||ND||ND||ND||ND||ND||ND||A|
|H3, H4, H5, H6, H9, H14, H22, H25, H26, H30, H35, H38, H49, H50, H53, H55, H57, H58, H63, H65, H67, H68, H69, H71, H72, H73, H79||+||–||+||–||–||–||–||+||–||ND||ND||ND||ND||ND||ND||B|
|H1, H7, H8, H10, H13, H15, H17, H18, H19, H20, H28, H29, H33, H34, H36, H43, H44, H45, H46, H47, H51, H59||+||–||–||+||–||–||–||–||+||ND||ND||ND||ND||ND||ND||C|
*ND, not done; NT, nontypeable.
†We performed PCR capsular typing using the oligonucleotides given in Technical Appendix Table). Results were interpreted as done previously (7): isolates positive for PCR ABC, A, and B were typed as A, isolates positive for PCR ABC and B were typed as B, and isolates positive for PCR ABC and C were typed as C.
‡Western blot analyses on polysaccharidic structures were performed by using specific polyclonal rabbit antisera.
- Butler T. Capnocytophaga canimorsus: an emerging cause of sepsis, meningitis, and post-splenectomy infection after dog bites. Eur J Clin Microbiol Infect Dis. 2015;34:1271–80.
- Butler T, Weaver RE, Ramani TK, Uyeda CT, Bobo RA, Ryu JS, et al. Unidentified gram-negative rod infection. A new disease of man. Ann Intern Med. 1977;86:1–5.
- Pers C, Gahrn-Hansen B, Frederiksen W. Capnocytophaga canimorsus septicemia in Denmark, 1982-1995: review of 39 cases. Clin Infect Dis. 1996;23:71–5.
- van Dam AP, Jansz A. Capnocytophaga canimorsus infections in The Netherlands: a nationwide survey. Clin Microbiol Infect. 2011;17:312–5.
- Hästbacka J, Hynninen M, Kolho E. Capnocytophaga canimorsus bacteremia: clinical features and outcomes from a Helsinki ICU cohort. Acta Anaesthesiol Scand. 2016;60:1437–43.
- Renzi F, Ittig SJ, Sadovskaya I, Hess E, Lauber F, Dol M, et al. Evidence for a LOS and a capsular polysaccharide in Capnocytophaga canimorsus. Sci Rep. 2016;6:38914.
- Hess E, Renzi F, Koudad D, Dol M, Cornelis GR. Identification of virulent Capnocytophaga canimorsus isolates by capsular typing. J Clin Microbiol. 2017;55:1902–14.
- Owren PA. Thrombotest. A new method for controlling anticoagulant therapy. Lancet. 1959;2:754–8.
- Tissari P, Zumla A, Tarkka E, Mero S, Savolainen L, Vaara M, et al. Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study. Lancet. 2010;375:224–30.
- Renzi F, Dol M, Raymackers A, Manfredi P, Cornelis GR. Only a subset of C. canimorsus strains is dangerous for humans. Emerg Microbes Infect. 2016;5:e29.
- Mally M, Shin H, Paroz C, Landmann R, Cornelis GR. Capnocytophaga canimorsus: a human pathogen feeding at the surface of epithelial cells and phagocytes. PLoS Pathog. 2008;4:e1000164.
- Manfredi P, Renzi F, Mally M, Sauteur L, Schmaler M, Moes S, et al. The genome and surface proteome of Capnocytophaga canimorsus reveal a key role of glycan foraging systems in host glycoproteins deglycosylation. Mol Microbiol. 2011;81:1050–60.
- Renzi F, Manfredi P, Mally M, Moes S, Jenö P, Cornelis GR. The N-glycan glycoprotein deglycosylation complex (Gpd) from Capnocytophaga canimorsus deglycosylates human IgG. PLoS Pathog. 2011;7:e1002118.
- Suzuki M, Kimura M, Imaoka K, Yamada A. Prevalence of Capnocytophaga canimorsus and Capnocytophaga cynodegmi in dogs and cats determined by using a newly established species-specific PCR. Vet Microbiol. 2010;144:172–6.
1These first authors contributed equally to this article.
2These authors were co–principal investigators.