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Volume 24, Number 12—December 2018
Research Letter

Use of Next-Generation Sequencing for Diagnosis of West Nile Virus Infection in Patient Returning to Belgium from Hungary

Elke Wollants1Comments to Author , David Smolders1, Reinout Naesens, Peggy Bruynseels, Katrien Lagrou, Jelle Matthijnssens, and Marc Van Ranst
Author affiliations: KU Leuven Rega Institute, Rega Institute, Leuven, Belgium (E. Wollants, J. Matthijnssens, M. Van Ranst); ZNA Hospital Middelheim, Antwerp, Belgium (D. Smolders, R. Naesens, P. Bruynseels); University Hospital Leuven, Leuven, Belgium (K. Lagrou, M. Van Ranst)

Main Article

Figure

Alignments of complete WNV genome identified from patient in Belgium compared with 32 known reference strains from the 5 established WNV lineages. A phylogenetic tree was constructed using the maximum-likelihood method based on the Tamura-Nei model. Evolutionary analyses were conducted in MEGA7 (http://www.megasoftware.net). Bootstrapping was conducted with 1,000 bootstrap replicates; only bootstraps values over 70% are shown. The phylogenetic tree clusters the strain from this patient together

Figure. Alignments of complete WNV genome identified from patient in Belgium compared with 32 known reference strains from the 5 established WNV lineages. A phylogenetic tree was constructed using the maximum-likelihood method based on the Tamura-Nei model. Evolutionary analyses were conducted in MEGA7 (http://www.megasoftware.net). Bootstrapping was conducted with 1,000 bootstrap replicates; only bootstrap values over 70% are shown. The phylogenetic tree clusters the strain from this patient together with other WNV strains from southeastern Europe in lineage II. The patient isolate, named WNV-2|Belgium|2017|Antwerp (GenBank accession no. MH021189), is most similar to a strain from Hungary. GenBank accession numbers are provided for reference isolates. Scale bar represents genetic distance.

Main Article

1These authors contributed equally to this article.

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