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Volume 24, Number 12—December 2018
Research

Novel Type of Chronic Wasting Disease Detected in Moose (Alces alces), Norway

Laura Pirisinu, Linh Tran, Barbara Chiappini, Ilaria Vanni, Michele A. Di Bari, Gabriele Vaccari, Turid Vikøren, Knut Ivar Madslien, Jørn Våge, Terry Spraker, Gordon Mitchell, Aru Balachandran, Thierry Baron, Cristina Casalone, Christer M. Rolandsen, Knut H. Røed, Umberto Agrimi, Romolo Nonno, and Sylvie L. BenestadComments to Author 
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, B. Chiappini, I. Vanni, M.A. Di Bari, G. Vaccari, U. Agrimi, R. Nonno); Norwegian Veterinary Institute, Oslo, Norway (L. Tran, T. Vikøren, K.I. Madslien, J. Våge, S.L. Benestad); Colorado State University, Fort Collins, Colorado, USA (T. Spraker); Canadian Food Inspection Agency, Ottawa, Ontario, Canada (G. Mitchell, A. Balachandran); Anses Lyon Unité “Maladies Neuro-Dégénératives,” Lyon, France (T. Baron); Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta, Torino, Italy (C. Casalone); Norwegian Institute for Nature Research, Trondheim, Norway (C.M. Rolandsen); Norwegian University of Life Sciences, Faculty of Veterinary Science, Oslo (K.H. Røed)

Main Article

Figure 2

Western blot analysis of PrPres in brains of chronic wasting disease-affected cervids from Norway and Canada. A) Western blot analysis PrPres in brains of moose and reindeer from Norway. Membrane was probed with L42 monoclonal antibodies. Molecular weights (kDa) are indicated on the right. Tissue equivalent loaded per lane was 1 mg. B) Western blot analysis of PrPres from moose isolates from Norway (lanes 6–7) compared with PrPres from chronic wasting disease–affected elk or wapiti (lanes 1–3),

Figure 2. Western blot analysis of PrPres in brains of chronic wasting disease–affected cervids from Norway and Canada. A) Western blot analysis PrPres in brains of moose and reindeer from Norway. Membrane was probed with L42 monoclonal antibodies. Molecular weights (kDa) are indicated on the right. Tissue equivalent loaded per lane was 1 mg. B) Western blot analysis of PrPres from moose isolates from Norway (lanes 6–7) compared with PrPres from chronic wasting disease–affected elk or wapiti (lanes 1–3), white-tailed deer (lane 4), and moose (lane 5) from Canada. Membranes were probed with L42 (upper) and 12B2 (lower) monoclonal antibodies. A scrapie sheep sample from Italy was added as control (lane 8). Molecular weights (kDa) are indicated on the right of each blot. Tissue equivalents loaded per lane were 1 mg for Canadian isolates, 2 mg for Norwegian isolates, and 0.15 mg for scrapie sheep control. PrPres, protease-resistant core of abnormal form of prion protein.

Main Article

Page created: November 20, 2018
Page updated: November 20, 2018
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