Figure 3. Characterization of PrPres fragments from moose (Alces alces) in Europe by epitope mapping. Mapping with mAbs spanning the whole prion protein enabled the analysis of PrPres in moose samples before (PNGase F–) and after (PNGase F+) deglycosylation, based on presence or absence of the epitopes and apparent molecular weight. Lanes 1, moose no. 1; lanes 2, moose no. 3; lane M, protein standards; lane 3, sheep scrapie sample. Solid arrowheads indicate C-terminal fragment of ≈13 kDa fragment (present in both samples and detected with SAF84 mAbs). Open arrowheads indicate C-terminal fragment of ≈16 kDa fragment in moose no. 2 with SAF84 and L42 mAbs. Asterisk indicates the internal fragment detected in moose no. 1 with 9A2 mAbs. Molecular weights are indicated on the left. In the blots on the right, protein standards are shown in lane M (10, 15, 20, 25, and 37 kDa). The mAbs used are indicated on the right. mAbs, monoclonal antibodies; PrPres, protease-resistant core of abnormal form of prion protein.