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Volume 24, Number 12—December 2018
Research

Novel Type of Chronic Wasting Disease Detected in Moose (Alces alces), Norway

Laura Pirisinu, Linh Tran, Barbara Chiappini, Ilaria Vanni, Michele A. Di Bari, Gabriele Vaccari, Turid Vikøren, Knut Ivar Madslien, Jørn Våge, Terry Spraker, Gordon Mitchell, Aru Balachandran, Thierry Baron, Cristina Casalone, Christer M. Rolandsen, Knut H. Røed, Umberto Agrimi, Romolo Nonno, and Sylvie L. BenestadComments to Author 
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, B. Chiappini, I. Vanni, M.A. Di Bari, G. Vaccari, U. Agrimi, R. Nonno); Norwegian Veterinary Institute, Oslo, Norway (L. Tran, T. Vikøren, K.I. Madslien, J. Våge, S.L. Benestad); Colorado State University, Fort Collins, Colorado, USA (T. Spraker); Canadian Food Inspection Agency, Ottawa, Ontario, Canada (G. Mitchell, A. Balachandran); Anses Lyon Unité “Maladies Neuro-Dégénératives,” Lyon, France (T. Baron); Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta, Torino, Italy (C. Casalone); Norwegian Institute for Nature Research, Trondheim, Norway (C.M. Rolandsen); Norwegian University of Life Sciences, Faculty of Veterinary Science, Oslo (K.H. Røed)

Main Article

Figure 3

Characterization of PrPres fragments from moose (Alces alces) in Europe by epitope mapping. Mapping with mAbs spanning the whole prion protein enabled the analysis of PrPres in moose samples before (PNGase F–) and after deglycosylation (PNGase F+), based on presence or absence of the epitopes and apparent molecular weight. Solid arrowheads indicate C-terminal fragment of ≈13 kDa fragment (present in both samples and detected with SAF84 mAbs). Open arrowheads indicate C-terminal fragment of ≈16 k

Figure 3. Characterization of PrPres fragments from moose (Alces alces) in Europe by epitope mapping. Mapping with mAbs spanning the whole prion protein enabled the analysis of PrPres in moose samples before (PNGase F–) and after (PNGase F+) deglycosylation, based on presence or absence of the epitopes and apparent molecular weight. Lanes 1, moose no. 1; lanes 2, moose no. 3; lane M, protein standards; lane 3, sheep scrapie sample. Solid arrowheads indicate C-terminal fragment of ≈13 kDa fragment (present in both samples and detected with SAF84 mAbs). Open arrowheads indicate C-terminal fragment of ≈16 kDa fragment in moose no. 2 with SAF84 and L42 mAbs. Asterisk indicates the internal fragment detected in moose no. 1 with 9A2 mAbs. Molecular weights are indicated on the left. In the blots on the right, protein standards are shown in lane M (10, 15, 20, 25, and 37 kDa). The mAbs used are indicated on the right. mAbs, monoclonal antibodies; PrPres, protease-resistant core of abnormal form of prion protein.

Main Article

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Page updated: November 20, 2018
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