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Volume 24, Number 12—December 2018
Research

Novel Type of Chronic Wasting Disease Detected in Moose (Alces alces), Norway

Laura Pirisinu, Linh Tran, Barbara Chiappini, Ilaria Vanni, Michele A. Di Bari, Gabriele Vaccari, Turid Vikøren, Knut Ivar Madslien, Jørn Våge, Terry Spraker, Gordon Mitchell, Aru Balachandran, Thierry Baron, Cristina Casalone, Christer M. Rolandsen, Knut H. Røed, Umberto Agrimi, Romolo Nonno, and Sylvie L. BenestadComments to Author 
Author affiliations: Istituto Superiore di Sanità, Rome, Italy (L. Pirisinu, B. Chiappini, I. Vanni, M.A. Di Bari, G. Vaccari, U. Agrimi, R. Nonno); Norwegian Veterinary Institute, Oslo, Norway (L. Tran, T. Vikøren, K.I. Madslien, J. Våge, S.L. Benestad); Colorado State University, Fort Collins, Colorado, USA (T. Spraker); Canadian Food Inspection Agency, Ottawa, Ontario, Canada (G. Mitchell, A. Balachandran); Anses Lyon Unité “Maladies Neuro-Dégénératives,” Lyon, France (T. Baron); Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d’Aosta, Torino, Italy (C. Casalone); Norwegian Institute for Nature Research, Trondheim, Norway (C.M. Rolandsen); Norwegian University of Life Sciences, Faculty of Veterinary Science, Oslo (K.H. Røed)

Main Article

Figure 5

Comparison of protease-resistant PrPres from moose (Alces alces) with chronic wasting disease and from sheep with scrapie, Europe. Representative blots show epitope mapping analysis ofPrPres (lane 4, CH1641; lane 5, moose no. 1; lane 6, moose no. 2) in comparison with different ovine transmissible spongiform encephalopathy isolates (lane 1, atypical/Nor98; lane 2, classical scrapie; and lane 3, CH1641). A chronic wasting disease isolate from Canada was loaded as control (lane 7). The antibodies

Figure 5. Comparison of protease-resistant PrPres from moose (Alces alces) with chronic wasting disease and from sheep with scrapie, Europe. Representative blots show epitope mapping analysis of PrPres (lane 4, CH1641; lane 5, moose no. 1; lane 6, moose no. 2) in comparison with different ovine transmissible spongiform encephalopathy isolates (lane 1, atypical/Nor98; lane 2, classical scrapie; and lane 3, CH1641). A chronic wasting disease isolate from Canada was loaded as control (lane 7). The antibodies used are indicated on the left. Protein standards are shown in lane M (10, 15, 20, 25, 37, and 50 kDa). The small amount of PrPres with intact 12B2 epitope in moose no.1 had a molecular weight higher than that observed with more C-terminal monoclonal antibodies (18.7 +0.3 kDa measured with 12B2 vs. 17.2 +0.1 kDa measured with L42). Even if the increase of the apparent molecular weight might be a known behavior when proteinase K cleavage occurs near the epitope, we noted that, in the case of moose no. 1, the 12B2-positive PrPres had a molecular weight higher than scrapie (18.1 +0.1 kDa measured with 12B2) and CH1641-like sample (18.1 +0.4 kDa when detected with 12B2). PrPres, protease-resistant core of abnormal form of prion protein.

Main Article

Page created: November 20, 2018
Page updated: November 20, 2018
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