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Volume 24, Number 12—December 2018
Research

Rat Hepatitis E Virus as Cause of Persistent Hepatitis after Liver Transplant

Siddharth Sridhar, Cyril C.Y. Yip, Shusheng Wu, Jianpiao Cai, Anna Jin-Xia Zhang, Kit-Hang Leung, Tom W.H. Chung, Jasper F.W. Chan, Wan-Mui Chan, Jade L.L. Teng, Rex K.H. Au-Yeung, Vincent C.C. Cheng, Honglin Chen, Susanna K.P. Lau, Patrick C.Y. Woo, Ning-Shao Xia, Chung-Mau Lo, and Kwok-Yung YuenComments to Author 
Author affiliations: The University of Hong Kong, Hong Kong, China (S. Sridhar, C.C.Y. Yip, S. Wu, J. Cai, A.J.-X. Zhang, K.-H. Leung, T.W.H. Chung, J.F.W. Chan, W.-M. Chan, J.L.L. Teng, R.K.H. Au-Yeung, V.C.C. Cheng, H. Chen, S.K.P. Lau, P.C.Y. Woo, C.-M. Lo, K.-Y. Yuen); The University of Hong Kong–Shenzhen Hospital, Shenzhen, China (J.F.W. Chan, C.-M. Lo, K.-Y. Yuen); Xiamen University, Xiamen, China (N.-S. Xia)

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Figure 2

Serologic testing for HEV infection at Queen Mary Hospital, Hong Kong. A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis gel showing purified HEV-A and HEV-C 239-aa recombinant proteins used in Western blot and ELISA. Lane 1, molecular weight marker; lane 2, HEV-A protein; lane 3, HEV-C protein. B–C) IgM and IgG Western blot using HEV-A protein (B) and HEV-C protein (C). Lane 1, patient serum (posttransplant day 100); lanes 2–5, individual platelet donor serum; lane 6, organ donor ser

Figure 2. Serologic testing for HEV infection at Queen Mary Hospital, Hong Kong. A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis gel showing purified HEV-A and HEV-C 239-aa recombinant proteins used in Western blot and ELISA. Lane 1, molecular weight marker; lane 2, HEV-A protein; lane 3, HEV-C protein. B–C) IgM and IgG Western blot using HEV-A protein (B) and HEV-C protein (C). Lane 1, patient serum (posttransplant day 100); lanes 2–5, individual platelet donor serum; lane 6, organ donor serum; lane 7, murine polyclonal serum against HEV-C; lane 8, specific monoclonal antibody against HEV-A; lane 9, cross-reactive monoclonal antibody against HEV-A and HEV-C. D, E) HEV-A and HEV-C ELISA IgG titers of patient pretransplant (D) and posttransplant serum (E) using an OD of 0.3 as assay cutoff as described in the Technical Appendix. HEV, hepatitis E virus; HEV-A, Orthohepevirus A; HEV-C, Orthohepevirus C; OD, optical density.

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Page created: November 20, 2018
Page updated: November 20, 2018
Page reviewed: November 20, 2018
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