Volume 24, Number 12—December 2018
Using PCR-Based Sequencing to Diagnose Haycocknema perplexum Infection in Human Myositis Case, Australia
|Designation||Primer pair||Oligonucleotide sequence, 5′ → 3′||Annealing temperature, °C||Expected size, bp||Reference|
|2° PCR||HPCO1F||GGACATCCTGAGGTGTATAT||This study|
*All PCR used 35 cycles with initial extension of 5 min. All cycles were 30 s except that SSU had a 1 min extension for PCRs. cox-1, cytochrome c oxidase 1; SSU, small subunit of nuclear ribosomal RNA.
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