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Volume 24, Number 2—February 2018
CME ACTIVITY - Research

Clinical and Molecular Epidemiology of Staphylococcal Toxic Shock Syndrome in the United Kingdom

Hema Sharma, Debra Smith, Claire E. Turner1, Laurence Game, Bruno Pichon, Russell Hope, Robert Hill, Angela Kearns2, and Shiranee Sriskandan2Comments to Author 
Author affiliations: Imperial College, London, UK (H. Sharma, D. Smith, C.E. Turner, S. Sriskandan); MRC London Institute of Medical Sciences, London (L. Game); Public Health England, London (B. Pichon, R. Hope, R. Hill, A. Kearns)

Main Article

Figure 4

TSST-1 and total mitogen production in vitro by tst-positive clonal complex (CC) 30 MSSA and CC30 MRSA strains. A) Mean TSST-1 present in the culture supernatants of tst-positive CC30 MSSA (n = 81) and CC30 MRSA (n = 39) isolates measured by immunoblot after overnight culture in brain–heart infusion broth. B) Mean human PBMC proliferative response to culture supernatants of tst-positive CC30 MSSA (n = 77) and CC30 MRSA (n = 39) isolates. Negative indicates RPMI tissue culture medium (Invitrogen,

Figure 4. TSST-1 and total mitogen production in vitro by tst-positive clonal complex (CC) 30 MSSA and CC30 MRSA strains. A) Mean TSST-1 present in the culture supernatants of tst-positive CC30 MSSA (n = 81) and CC30 MRSA (n = 39) isolates measured by immunoblot after overnight culture in brain–heart infusion broth. B) Mean human PBMC proliferative response to culture supernatants of tst-positive CC30 MSSA (n = 77) and CC30 MRSA (n = 39) isolates. Negative indicates RPMI tissue culture medium (Invitrogen, Hemel Hempstead, UK) alone. Error bars indicate SDs. *p<0.05; ***p<0.0001 (both by 2-tailed t-test). cpm, counts per minute; MSSA, methicillin-sensitive S. aureus; MRSA, methicillin-resistant S. aureus; PBMC, peripheral blood mononuclear cells; TSS, toxic shock syndrome; TSST-1, TSS toxin 1.

Main Article

1Current affiliation: University of Sheffield, Sheffield, UK.

2These authors contributed equally to this article.

Page created: January 11, 2018
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