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Volume 24, Number 2—February 2018
Research

Multiplex PCR−Based Next-Generation Sequencing and Global Diversity of Seoul Virus in Humans and Rats

Won-Keun Kim1, Jin Sun No1, Seung-Ho Lee, Dong Hyun Song, Daesang Lee, Jeong-Ah Kim, Se Hun Gu, Sunhye Park, Seong Tae Jeong, Heung-Chul Kim, Terry A. Klein, Michael R. Wiley, Gustavo Palacios, and Jin-Won SongComments to Author 
Author affiliations: Korea University, Seoul, South Korea (W.-K. Kim, J.S. No, S.-H. Lee, J.-A. Kim, J.-W. Song); Agency for Defense Development, Daejeon, South Korea (D.H. Song, D. Lee, S.H. Gu, S. Park, S.T. Jeong); 65th Medical Brigade/Medical Department Activity−Korea, Seoul (H.-C. Kim, T.A. Klein); US Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA (M.R. Wiley, G. Palacios)

Main Article

Table 1

Laboratory diagnosis of samples from patients with SEOV-induced HFRS, South Korea, 2002*

Sample Onset date Collection date IFA titer† Reverse transcription quantitative PCR result‡
SEOV Hu02-112 Unknown Unknown 1:128 –
SEOV Hu02-180 2002 Feb 9 2002 Feb 15 1:1,024 +
SEOV Hu02-258 2002 Feb 28 2002 Mar 7 1:4,096 +
SEOV Hu02-294 Unknown Unknown 1:4,096 –
SEOV Hu02-529 2002 May 8 2002 May 13 1:2,048 –
SEOV Hu02-668 2002 May 30 2002 Jun 18 1:128 –

*HFRS, hemorrhagic fever with renal syndrome; Hu, human; IFA, indirect immunofluorescence antibody test; SEOV, Seoul virus; +, positive; –, negative.
†For IgG against SEOV. Antibody titration was tested by using serial 2-fold dilutions starting at a serum dilution of 1:32.
‡For large segment RNA using hantavirus genus–reactive primers.

Main Article

1These authors contributed equally to this article.

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Page updated: January 17, 2018
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