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Volume 24, Number 2—February 2018
Research

Macacine Herpesvirus 1 Antibody Prevalence and DNA Shedding among Invasive Rhesus Macaques, Silver Springs State Park, Florida, USA

Samantha M. WiselyComments to Author , Katherine A. Sayler, C. Jane Anderson, Carisa L. Boyce, Amy R. Klegarth, and Steve A. Johnson
Author affiliations: University of Florida, Gainesville, Florida, USA (S.M. Wisely, K.A. Sayler, C.J. Anderson, C.L. Boyce, S.A. Johnson); University of Washington, Seattle, Washington, USA (A.R. Klegarth)

Main Article

Table 1

PCR oligonucleotide primers and probe used in the detection of McHV-1 viral DNA in samples from rhesus macaques and soil, Silver Springs State Park, Florida, USA, 2000–2012*

PCR target Reference Sequence, 5′ → 3′
gGBV-323F (21) TGGCCTACTACCGCGTGG
gGBV-446R (21) TGGTACGTGTGGGAGTCGCG
gGBV-403T (21) (6-FAM)CCGCCCTCTCCGAGCACGTG(BHQ-1)
DFA (22) GAYTTYGCNAGYYTNTAYCC
ILK (22) TCCTGGACAAGCAGCARNYSGCNMTNAA
KG1 (22) GTCTTGCTCACCAGNTCNACNCCYTT
TGV (22) TGTAACTCGGTGTAYGGNTTYACNGGNGT
IYG (22) CACAGAGTCCGTRTCNCCRTADAT
HB2A (8) CCGCGCTCGCCACGGACACCA
HB2B (8) ATCGCGCGCCGGACCGATCGT
AS9 (23) TC[A/T]CCCGGGCTAGACTT[T/C][A/C]TCTTCCTGCTCAG
AS2 (23) ATGGCGGCCAGGGTCAGCGCGCAGAGG
AS8 (23) CTCTGCGCGCTGACCCTGGCCGCCATGG
AS7 (23) CACGTCGGGGGG[G/A]TCCGTCTTCTGCTCC

*BHQ-1, black hole quencher 1; 6-FAM, 6-fluorescein amidite; gG, glycoprotein G; McHV-1, macacine herpesvirus 1.

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Page updated: January 17, 2018
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