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Volume 24, Number 3—March 2018

Capsule Typing of Haemophilus influenzae by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry1

Viktor MånssonComments to Author , Janet R. Gilsdorf, Gunnar Kahlmeter, Mogens Kilian, J. Simon Kroll, Kristian Riesbeck2, and Fredrik Resman2
Author affiliations: Lund University, Malmö, Sweden (V. Månsson, K. Riesbeck, F. Resman); University of Michigan, Ann Arbor, Michigan, USA (J.R. Gilsdorf); Växjö Hospital, Växjö, Sweden (G. Kahlmeter); Aarhus University, Aarhus, Denmark (M. Kilian); Imperial College London, London, UK (J.S. Kroll)

Main Article

Table 2

Validation of the supplemented MALDI-TOF mass spectrometry capsule typing database (n = 50) by classification of 126 invasive isolates of Haemophilus influenzae from Sweden*

Capsule type No. tested No. correct† No inconclusive‡ No. incorrect§ Sensitivity, % Specificity, %
Hib 8 0 0 100 100
Hie 5 5 0 0 100 100
Hif 15 15# 0 0 100 100
All encapsulated isolates (b, e, and f) 28 28 0 0 100 100
NTHi 98 93 5 0 94.9 100

*Hib, H. influenzae type b; Hie, H. influenzae type e; Hif, H. influenza type f; MALDI-TOF, matrix-assisted laser desorption/ionization time-of-flight; ST, sequence type.
>5/6 spectra classified to the same correct type.
<4/6 spectra classified to the same type.
§>5/6 spectra classified to the same incorrect type.
¶All classified to the ST6-related Hib lineage.
#All classified to the established ST124-related Hif lineage.

Main Article

1Preliminary results from this study were presented at the IDWeek 2016 Conference, October 26−30, 2016, New Orleans, Louisiana, USA.

2These senior authors contributed equally to this article.

Page created: February 15, 2018
Page updated: February 15, 2018
Page reviewed: February 15, 2018
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