Volume 24, Number 4—April 2018
Research
Phenotypic and Genotypic Characterization of Enterobacteriaceae Producing Oxacillinase-48–Like Carbapenemases, United States
Joseph D. Lutgring
, Wenming Zhu, Tom J.B. de Man, Johannetsy J. Avillan, Karen F. Anderson, David R. Lonsway, Lori A. Rowe, Dhwani Batra, J. Kamile Rasheed, and Brandi M. Limbago
, Wenming Zhu, Tom J.B. de Man, Johannetsy J. Avillan, Karen F. Anderson, David R. Lonsway, Lori A. Rowe, Dhwani Batra, J. Kamile Rasheed, and Brandi M. Limbago
Table 1
Sequences of primers and probes used for identification of Enterobacteriaceae isolates with β-lactamase OXA-48−like carbapenemases, United States*
| Primers and probes | Sequence, 5′→3′ |
|---|---|
| 16S rRNA, forward primer | TGG AGC ATG TGG TTT AAT TCG A |
| 16S rRNA, reverse primer | TGC GGG ACT TAA CCC AAC A |
| 16S rRNA, probe (CY5) | CY5-CA CGA GCT GAC GAC ARC CAT GCA-BHQ |
| OXA-48, forward 180 | ACG GGC GAA CCA AGC AT |
| OXA-48, reverse 239 | GCG ATC AAG CTA TTG GGA ATT T |
| OXA-48, probe 199 | FAM-TT ACC CGC ATC TAC C-BHQ |
| OXA-48, forward 722 | TGC CCA CAT CGG ATG GTT |
| OXA-48, reverse 781 | CCT GTT TGA GCA CTT CTT TTG TGA |
| OXA-48, probe 741 | AG GGC TGC GCC AAG |
| OXA-48 F1 | ATG CGT GTR TTA GCC TTA TC |
| OXA-48 R1 | CTA KGG AAT WAT YTT YTC CTG |
*OXA, oxacillinase.
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Page updated: July 31, 2018
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