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Volume 24, Number 5—May 2018

Dynamics of Spirochetemia and Early PCR Detection of Borrelia miyamotoi

Lyudmila Karan, Marat MakenovComments to Author , Nadezhda Kolyasnikova, Olga Stukolova, Marina Toporkova, and Olga Olenkova
Author affiliations: Central Research Institute of Epidemiology, Moscow, Russia (L. Karan, M. Makenov, N. Kolyasnikova, O. Stukolova); Medical Association Novaya Bolnitsa, Yekaterinburg, Russia (M. Toporkova); Clinical Diagnostic Center, Yekaterinburg (O. Olenkova)

Main Article

Table 1

Cross-validation of detection of Borrelia spp. by quantitative PCR and ELISA in blood samples from 310 patients with suspected borreliosis, Yekaterinburg, Russia, 2010 and 2015*

Quantitative PCR for 16S rRNA gene
No. patients
No. ELISA positive
No. ELISA negative
With erythema migrans
Borrelia burgdorferi sensu lato 31 30 1 0
B. miyamotoi 5 5 0 0
PCR negative 131 81 39 11
Without erythema migrans
B. burgdorferi sensu lato 4 4 0 0
B. miyamotoi 65 55 10 0
B. burgdorferi sensu lato plus B. miyamotoi 1 0 1 0
PCR negative 73 73 0 0
Total 143 132 11 0

*ELISA results are for borrelial IgM or IgG seroconversion. ND, not determined.

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Page created: April 17, 2018
Page updated: April 17, 2018
Page reviewed: April 17, 2018
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