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Volume 24, Number 8—August 2018
Dispatch

Direct Detection of penA Gene Associated with Ceftriaxone-Resistant Neisseria gonorrhoeae FC428 Strain by Using PCR

David M. WhileyComments to Author , Lebogang Mhango, Amy V. Jennison, Graeme Nimmo, and Monica M. Lahra
Author affiliations: The University of Queensland, Brisbane, Queensland, Australia (D.M. Whiley, L. Mhango); Pathology Queensland Central Laboratory, Brisbane (D.M. Whiley, G. Nimmo); Queensland Health Forensic and Scientific Services, Archerfield, Queensland, Australia (A.V. Jennison); Griffith University, Gold Coast, Queensland, Australia (G. Nimmo); The Prince of Wales Hospital Randwich, Sydney, New South Wales, Australia (M.M Lahra); The University of New South Wales, Sydney (M.M. Lahra)

Main Article

Figure

Sequence alignment showing the expected 112-bp PCR product for the PCR to detect Neisseria gonorrhoeae FC428 strain. PenA type 60.001 is provided as the reference sequence. Gray indicates the primer targets and the 311 codon within the probe target sequences. The penA sequences from the N. gonorrhoeae A8806, N. meningitidis, and N. lactamica isolates that cross-reacted with the FC428 PCR are also provided.

Figure. Sequence alignment showing the expected 112-bp PCR product for the PCR to detect Neisseria gonorrhoeae FC428 strain. PenA type 60.001 is provided as the reference sequence. Gray indicates the primer targets and the 311 codon within the probe target sequences. The penA sequences from the N. gonorrhoeae A8806, N. meningitidis, and N. lactamica isolates that cross-reacted with the FC428 PCR are also provided. Dots indicate sequence identity.

Main Article

Page created: July 18, 2018
Page updated: July 18, 2018
Page reviewed: July 18, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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