Figure 2. Analysis of an orthopoxvirus isolated from an infected cat, Italy. A) Cytoplasmic fluorescence in infected Vero cells using serum from the diseased cat (original magnification ×400). B) Cytoplasmic inclusion bodies (arrows) in infected Vero cells (hematoxylin and eosin stain, original magnification ×400). C) Pocks (arrows) in the inoculated chorioallantoic membrane of a 12-day-old chick embryo. D) Electron micrograph of orthopoxvirus-like particle from infected Vero cells. The virus preparation was negative-stained with sodium phosphotungstate (original magnification ×25,000).