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Volume 25, Number 1—January 2019
Dispatch

Dengue Virus IgM Serotyping by ELISA with Recombinant Mutant Envelope Proteins

Alexandra Rockstroh, Luisa Barzon, Widuranga Kumbukgolla, Hoang Xuan Su, Erley Lizarazo, Maria Fernanda Vincenti-Gonzalez, Adriana Tami, Alice M.M. Ornelas, Renato Santana Aguiar, Daniel Cadar, Jonas Schmidt-Chanasit, and Sebastian UlbertComments to Author 
Author affiliations: Fraunhofer Institute for Cell Therapy and Immunology, Leipzig, Germany (A. Rockstroh, S. Ulbert); University of Padova, Padova, Italy (L. Barzon); Rajarata University of Sri Lanka, Mihinthale, Sri Lanka (W. Kumbukgolla); Vietnam Military Medical University, Hanoi, Vietnam (H.X. Su); University Medical Center Groningen, Groningen, the Netherlands (E. Lizarazo, M.F. Vincenti-Gonzalez, A. Tami); Federal University of Rio de Janeiro, Rio de Janeiro, Brazil (A.M.M. Ornelas, R.S. Aguiar); Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (D. Cadar, J. Schmidt-Chanasit); German Centre for Infection Research, Hamburg (J. Schmidt-Chanasit)

Main Article

Table 1

Dominant serotype determined by DENV serotype–specific IgM ELISA and ELISA specificity, by cohort and serotype, 2013–2018*

Cohort, DENV serotype†
 No. samples
 Dominant serotype, no. (%) samples
 Serotype specificity‡
No. samples
 % Samples (95% CI)
Returning travelers
DENV-1 16 14 (87.50) 14 87.50 (61.65–98.45)
DENV-2 11 10 (90.91) 8 72.73 (39.03–93.98)
DENV-3 10 9 (90) 9 90.00 (55.50–99.75)
DENV-4 8 8 (100.00) 7 87.50 (47.35–99.68)
All serotypes
 45
 41 (91.11)
 38
 84.44 (70.54–93.51)
Residents in DENV-endemic countries
Sri Lanka
DENV-1 23 16 (69.57) 16 69.57 (47.08–86.79)
DENV-2 14 13 (92.86) 13 92.86 (66.13–99.82)
DENV-4 6 6 (100.00) 6 100.00 (54.07–100.00)
All serotypes 43 35 (81.40) 35 81.40 (66.60–91.61)
Vietnam
DENV-1 20 19 (95.00) 19 95.00 (75.13–99.87)
DENV-2 4 4 (100.00) 4 100.00 (39.76–100.00)
All serotypes 24 23 (95.83) 23 95.83 (78.88–99.89)
Venezuela
DENV-4 5 4 (80.00) 4 80.00 (23.36–99.49)
Brazil
DENV-4 2 2 (100.00) 2 100.00 (15.81–100.00)
Total
DENV-1 43 35 (81.40) 35 81.40 (66.60–91.61)
DENV-2 18 17 (94.44) 17 94.44 (72.71–99.86)
DENV-4 13 12 (92.31) 12 92.31 (76.55–99.81)
All serotypes
 74
 64 (86.49)
 64
 86.49 (76.55–93.32)
Total, all serotypes 119 105 (88.24) 102 85.71 (77.12–91.45)

*DENV, dengue virus.
†Determined by PCR.
‡Determined by correspondence with PCR results.

Main Article

Page created: December 18, 2018
Page updated: December 18, 2018
Page reviewed: December 18, 2018
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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