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Volume 25, Number 1—January 2019

Association of Increased Receptor-Binding Avidity of Influenza A(H9N2) Viruses with Escape from Antibody-Based Immunity and Enhanced Zoonotic Potential

Joshua E. Sealy, Tahir Yaqub, Thomas P. Peacock1, Pengxiang Chang, Burcu Ermetal, Anabel Clements, Jean-Remy Sadeyen, Arslan Mehboob2, Holly Shelton, Juliet E. Bryant, Rod S. Daniels, John W. McCauley, Munir IqbalComments to Author , and Jean-Remy Royal Veterinary CollegeLondonUKSadeyen
Author affiliations: The Pirbright Institute, Pirbright, UK (J.E. Sealy, T.P. Peacock, P. Chang, A. Clements, J.-R. Sadeyen, H. Shelton, M. Iqbal); University of Veterinary and Animal Sciences, Lahore, Pakistan (T. Yaqub, A. Mehboob); The Francis Crick Institute, London (B. Ermetal, R.S. Daniels, J.W. McCauley); Fondation Mérieux, Lyon, France (J.E. Bryant)

Main Article

Table 1

Change in HI titers of influenza A(H9N2) viruses from Pakistan during 2014–2016 viruses compared to UDL-01/08 virus*

HA backbone and substitution Fold change in HI titer (titer)†
I116L – (2,048)
P118S – (2,048)
S134L – (2,048)
N135D – (2,048)
N148S – (2,048)
A156V 1‡ (4,096)
R162Q + D262N – (2,048)
G163E – (2,048)
K164N 1‡ (4,096)
180T 4 (128)
A180T + K164N 4 (128)
A180V 4 (128)
N198D (2,048)
D262N (2,048)
1‡ (4,096)
A180T 4 (128)
A180V 4 (128)

*Postinfection polyclonal antiserum raised in a single chicken inoculated with UDL-01/08 was used in all HI assays. Amino acid substitutions were introduced into a contemporary virus HA backbone (SKP-827/16 with A180) or the UDL-01/08 HA backbone and rescued by reverse genetics. HI titers between contemporary viruses and UDL-01/08 virus were compared. HA, hemagglutinin; HI, hemagglutination inhibition; –, no change.
†The homologous HI titer for UDL-01/08 antiserum was 2,048.
‡Indicates fold decrease compared with UDL-01/08.

Main Article

1Current affiliation: Imperial College London, London, UK.

2Current affiliation: Chinese Academy of Agricultural Sciences, Beijing, China.

Page created: December 18, 2018
Page updated: December 18, 2018
Page reviewed: December 18, 2018
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