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Volume 25, Number 10—October 2019

Genetic Characterization and Zoonotic Potential of Highly Pathogenic Avian Influenza Virus A(H5N6/H5N5), Germany, 2017–2018

Anne Pohlmann1, Donata Hoffmann1, Christian Grund, Susanne Koethe, Daniela Hüssy, Simone M. Meier, Jacqueline King, Jan Schinköthe, Reiner Ulrich, Timm Harder, and Martin BeerComments to Author 
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (A. Pohlmann, D. Hoffmann, C. Grund, S. Koethe, J. King, J. Schinköthe, R. Ulrich, T. Harder, M. Beer); Institute of Virology and Immunology, Mittelhäusern, Switzerland (D. Hüssy); Vetsuisse Faculty Zurich, Zurich, Switzerland (S.M. Meier)

Main Article


Viral RNA loads in nasal washing samples from ferrets infected with highly pathogenic avian influenza A(H5N6/H5N5) clade virus isolates from Germany, 2017–2018, and seroconversion in study assessing virus zoonotic risk*

Group Day postinfection, viral RNA load, genome copies/µL
0 1 3 5 7 9

0.2 0.2 0.1 100.6 ND‡ +/−§
1.2 2.8 0.1 +
1.9 0.9 2.9 +
1.2 0.1 +



H5N6 0.8 1.3 1.3 +
0.2 1.9 13.8 3.2 +
0.2 0.2 0.2 +
0 1.3 0.2 4.2 +

*ND, not done; –, negative.
†Seroconversion measured with sensitive nucleoprotein ELISA (ID Screen Influenza A Antibody Competition ELISA Kit; ID.Vet, with day 14 or 7 serum sample, depending on day of animal sacrifice, and compared with preinoculation serum sample.
‡Sacrificed day 7 because animal displayed neurologic symptoms.
§Reaction interpreted as reactive but not positive.

Main Article

1These authors contributed equally to this article.

Page created: September 17, 2019
Page updated: September 17, 2019
Page reviewed: September 17, 2019
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