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Volume 25, Number 11—November 2019
Research Letter

Molecular Epidemiology of Hantaviruses in the Czech Republic

Hana ZelenaComments to Author , Petra Strakova, Marta Heroldova, Jakub Mrazek, Tomas Kastl, Alena Zakovska, Daniel Ruzek, Jan Smetana, and Ivo Rudolf
Author affiliations: Institute of Public Health in Ostrava, Ostrava, Czech Republic (H. Zelena, J. Mrazek); University of Defence, Hradec Kralove, Czech Republic (H. Zelena, J. Smetana); The Czech Academy of Sciences, Brno, Czech Republic (P. Strakova, M. Heroldova, I. Rudolf); Veterinary Research Institute, Brno (P. Strakova, T. Kastl, D. Ruzek); Mendel University, Brno (M. Heroldova); Masaryk University, Brno (A. Zakovska); Biology Centre of the Czech Academy of Sciences, Ceske Budejovice, Czech Republic (D. Ruzek)

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Figure

Phylogenetic tree constructed with partial 195-bp fragments of the DOBV large segment from humans and mice, Czech Republic, 2010–2018. Sequences from this study (bold) were compared with available sequences from the GenBank database; patient numbers are provided, and mouse samples are labeled. Samples with sequences identical to another sample were excluded for simplification purposes. Sequences were aligned with BioEdit (9), and the phylogenetic tree was prepared by using MEGA 7.0 (https://mega

Figure. Phylogenetic tree constructed with partial 195-bp fragments of the DOBV large segment from humans and mice, Czech Republic, 2010–2018. Sequences from this study (bold) were compared with available sequences from the GenBank database; patient numbers are provided, and mouse samples are labeled. Samples with sequences identical to another sample were excluded for simplification purposes. Sequences were aligned with BioEdit (9), and the phylogenetic tree was prepared by using MEGA 7.0 (https://megasoftware.net) and the neighbor-joining method. Analyses were performed with the Jukes-Cantor model by using a gamma distribution with 5 rate categories and a bootstrap value of 1,000. The genotype clusters are labeled. Scale bar indicates nucleotide substitutions per site. The Puumala virus sequence was used as an outgroup. DOBV, Dobrava-Belgrade virus.

Main Article

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