Volume 25, Number 2—February 2019
Research Letter
Little Evidence of Zika Virus Infection in Wild Long-Tailed Macaques, Peninsular Malaysia
Chong Long Chua, Yoke Fun Chan, Eva S.G. Soh Andu, Jeffrine J. Rovie-Ryan, Frankie Thomas Sitam, Khebir Verasahib, and I-Ching Sam
Table
Zika virus, DENV-1, and DENV-2 neutralization titers of serum samples collected from long-tailed macaques in Peninsular Malaysia, 2009, 2010, and 2016*
| Sample collection period and size |
No. samples† |
Macaque sex and age group, ID no. |
Town/city, state, coordinates |
Neutralization titers | |||
|---|---|---|---|---|---|---|---|
| Zika virus PRNT50 |
Zika virus FRNT50 |
DENV-1 FRNT50 |
DENV-2 FRNT50 |
||||
| October–November 2009 and October 2010, n = 145 |
1 |
Male adult, ZMW604 |
Bukit Serendah, Selangor, 3.36°N, 101.60°E |
640 |
640 |
<20 |
<20 |
| March and August 2016, n = 89 | 5 | Female juvenile, PMW804 | Manong, Perak, 4.61°N, 100.90°E | 40 | 20 | <20 | <20 |
| Female adult, WDSP/16/009 | Kuala Lipis, Pahang, 4.18°N, 102.05°E | 80 | 80 | <20 | 20 | ||
| Male adult, WDSP/16/006 | Kuala Lipis, Pahang, 4.18°N, 102.05°E | 80 | 80 | 640 | 160 | ||
| Male adult, WDSP/16/012 | Kuala Lipis, Pahang, 4.18°N, 102.05°E | 40 | 40 | 20 | 40 | ||
| Male adult, WDSP/16/086 | Batu Pahat, Johor, 1.85°N, 102.94°E | 40 | 20 | 40 | 20 | ||
*DENV-1, dengue virus serotype 1; DENV-2, dengue virus serotype 2; FRNT50, 50% focus reduction neutralization test; ID, identification; PRNT50, 50% plaque reduction neutralization test.
†Number of samples from the first batch (n = 234) that were positive by Zika virus PRNT50 and further tested by FRNT50.
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