Volume 25, Number 4—April 2019
Dispatch
Prolonged Shedding of Zika Virus RNA in Vaginal Secretions, Nicaragua
Yaoska Reyes1, Natalie M. Bowman1
, Sylvia Becker-Dreps, Edwing Centeno, Matthew H. Collins, Guei-Jiun Alice Liou, and Filemón Bucardo
, Sylvia Becker-Dreps, Edwing Centeno, Matthew H. Collins, Guei-Jiun Alice Liou, and Filemón Bucardo
Table
Clinical characteristics and viral shedding in 5 women with acute Zika virus infection, Nicaragua, 2016–2017*
| Characteristic | A013 | A018 | A020 | A024 | A039 |
|---|---|---|---|---|---|
| Age, y | 54 | 18 | 25 | 19 | 25 |
| Duration of symptoms at enrollment, d | 1 | 3 | 4 | 6 | 5 |
| Pregnant at enrollment | No | Yes | Yes | Yes | Yes |
| Estimated gestational age at enrollment, mo | NA | 7 | 7 | 5 | 1 |
| Timing of delivery, days after symptom onset | NA | 39 | 61 | 149 | 305 |
| Hematocrit, %, reference range 36–45 | 36 | 32 | 35 | 37 | 40 |
| Leukocytes, cells/mm3, reference range 5,000–10,000 | 4,230 | 5,410 | 3,510 | 17,430 | 8,050 |
| Sexual contact in the last 3 mo† | No | Yes | Yes | Yes | Yes |
| Previous infection with dengue virus† | Yes | No | No | No | No |
| Previous infection with chikungunya virus† | Yes | Yes | No | No | Yes |
| Fluid Zika-positive by qRT-PCR at baseline |
Saliva |
Blood, urine |
Blood |
Urine |
Blood, saliva |
| Vaginal fluid PCR results, Ct1/Ct2,‡ at approximate days after symptom onset | |||||
| 7 | NS | NS | NS | 0/0 | 0/0 |
| 14 | NS | 31/0 | 32/33 | NS | 0/0 |
| 21 | NS | 35/0 | 34/36 | 0/0 | 37/0 |
| 28 | 33/0 | 0/0 | NS | 0/0 | NS |
| 60 | NS | 34/36 | NS | 36/0 | NS |
| 90 | NS | 35/0 | NS | 0/0 | NS |
| 180 | NS | 37/0 | 0/0 | NS | NS |
*Ct, cycle threshold; NA, not applicable; NS, no sample; qRT-PCR, quantitative reverse transcription PCR.
†By participant report.
‡Ct1 refers to pan-Zika primers; Ct2 refers to Asian-specific primers.
1These authors contributed equally to this article.
Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.