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Volume 25, Number 4—April 2019
Dispatch

Anopheles sundaicus Mosquitoes as Vector for Plasmodium knowlesi, Andaman and Nicobar Islands, India

Pachalil Thiruvoth Vidhya, Ittoop Pulikkottil SunishComments to Author , Anwesh Maile, and Ali Khan Zahid
Author affiliations: ICMR Regional Medical Research Centre, Port Blair, Andaman and Nicobar Islands, India

Main Article

Figure 2

Agarose gel (2%) electrophoresis of nested PCR products of amplified Plasmodium knowlesi DNA sequences taken from mosquito samples (10 mosquitoes per pool) from Andaman and Nicobar Islands. A) Analysis of PCR products amplified with Pmk8 and Pmk9 primers. Lanes 1–4, pools of Anopheles sundaicus mosquitoes; lane 5, size marker DNA (100-bp DNA ladder); lanes 6 and 7, An. maculatus mosquito pools; lane 8, An. barbirostris mosquito pool (2 mosquitoes); lane 9, positive control; lane 10, negative con

Figure 2. Agarose gel (2%) electrophoresis of nested PCR products of amplified Plasmodium knowlesi DNA sequences taken from mosquito samples (10 mosquitoes per pool) from Andaman and Nicobar Islands. A) Analysis of PCR products amplified with Pmk8 and Pmk9 primers. Lanes 1–4, pools of Anopheles sundaicus mosquitoes; lane 5, size marker DNA (100-bp DNA ladder); lanes 6 and 7, An. maculatus mosquito pools; lane 8, An. barbirostris mosquito pool (2 mosquitoes); lane 9, positive control; lane 10, negative control. Arrows indicate positive sample from the An. sundaicus mosquito pool in lane 3, 500 bp marker in DNA ladder of lane 5, and positive control in lane 9. B) Analysis of nested PCR products amplified with PKF1140 and PKR1550 primers. Lanes 1–5, An. sundaicus mosquito pools; lanes 6–9, An. maculatus mosquito pools; lane 10, size marker DNA (100-bp DNA ladder); lane 11, An. maculatus mosquito pool; lane 12, An. barbirostris mosquito pool; lane 13, positive control; lane 14, negative control. Arrows indicate An. sundaicus mosquito pool with positive band at 410 bp in lane 3, 600-bp marker in DNA ladder in lane 10, and positive control of 410 bp in lane 13. PC, positive control.

Main Article

Page created: March 18, 2019
Page updated: March 18, 2019
Page reviewed: March 18, 2019
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