TY - JOUR AU - Steffen, Imke AU - Lu, Kai AU - Yamamoto, Lauren AU - Hoff, Nicole AU - Mulembakani, Prime AU - Wemakoy, Emile AU - Muyembe-Tamfum, Jean-Jacques AU - Ndembi, Nicaise AU - Brennan, Catherine AU - Hackett, John AU - Stramer, Susan AU - Switzer, William AU - Saragosti, Sentob AU - Mbensa, Guy AU - Laperche, Syria AU - Rimoin, Anne AU - Simmons, Graham T1 - Serologic Prevalence of Ebola Virus in Equatorial Africa T2 - Emerging Infectious Disease journal PY - 2019 VL - 25 IS - 5 SP - 911 SN - 1080-6059 AB - We conducted a serologic survey of 2,430 serum samples collected during 1997–2012 for various studies to determine the prevalence of the hemorrhagic fever virus Ebola virus (EBOV) in equatorial Africa. We screened serum samples for neutralizing antibodies by using a pseudotype microneutralization assay and a newly developed luciferase immunoprecipitation system assay. Specimens seroreactive for EBOV were confirmed by using an ELISA. Our results suggest a serologic prevalence of 2%–3.5% in the Republic of the Congo and the Democratic Republic of the Congo, which have reported outbreaks of infection with EBOV. In addition we detected a seroprevalence of 1.3% in southern Cameroon, which indicated a low risk for exposure in this region. KW - Ebola virus KW - viruses KW - hemorrhagic fever virus KW - folivirus KW - serologic prevalence KW - neutralization assay KW - pseudotypes KW - luciferase immunoprecipitation system KW - ELISA KW - equatorial Africa KW - filovirus KW - hemorrhagic fever KW - Republic of the Congo KW - Democratic Republic of the Congo KW - Cameroon KW - Uganda KW - Ghana DO - 10.3201/eid2505.180115 UR - https://wwwnc.cdc.gov/eid/article/25/5/18-0115_article ER - End of Reference