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Volume 25, Number 5—May 2019

Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues

Jennifer Chua1Comments to Author , Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. CoteComments to Author 
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA

Main Article

Table 1

Formaldehyde fixation of Bacillus anthracis Ames–infected skin tissue from guinea pigs in study of inactivation of bacterial Tier 1 Select Agents*

Fixation time, d, no. samples Maximum tissue weight, g Maximum tissue size, mm Total CFU recovered/g Sample inactivation† Positive control
7, 3 2.04 20 × 30 × 3 6.15 × 107 +/+‡ +/+
14, 3 1.94 23 × 28 × 3 5.41 × 107 –/– +/+
21, 3 2.55 28 × 28 × 3 4.86 × 107 –/– +/+

*Infected skin tissues from 3 guinea pigs were fixed for 7, 14, or 21 d in 4% paraformaldehyde, rinsed for 30 min in phosphate-buffered saline, and then homogenized. The maximum tissue weight, size, and CFU recovered per group are listed. +/– indicates presence or absence of growth in broth/on plates.
†100% of sample tested.
‡Of 3 samples, 1 was positive for growth.

Main Article

1These authors contributed equally to this article.

Page created: April 18, 2019
Page updated: April 18, 2019
Page reviewed: April 18, 2019
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