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Volume 25, Number 5—May 2019

Formaldehyde and Glutaraldehyde Inactivation of Bacterial Tier 1 Select Agents in Tissues

Jennifer Chua1Comments to Author , Joel A. Bozue1, Christopher P. Klimko, Jennifer L. Shoe, Sara I. Ruiz, Christopher L. Jensen, Steven A. Tobery, Jared M. Crumpler, Donald J. Chabot, Avery V. Quirk, Melissa Hunter, David E. Harbourt, Arthur M. Friedlander, and Christopher K. CoteComments to Author 
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Frederick, Maryland, USA

Main Article

Table 8

Formaldehyde fixation of infected lung tissue from rabbits exposed to aerosolized Bacillus anthracis Ames spores in study of inactivation of bacterial Tier I Select Agents*

Rabbit no. Tissue weight, g Tissue size, mm Total CFU recovered/g Heat-resistant CFU recovered/g Time of inactivation
Positive control
13 d 20 d
1 0.72 10 × 10 × 10 1.2 × 107 1.5 × 106 –/–† ND +/+
2 0.74 10 × 10 × 10 4.4 × 105 4.3 × 105 –/–† ND +/+
3 0.71/0.75 10 × 10 × 10 2.6 × 106 1.0 × 106 +/+† –/–† +/+

*Infected lung tissues from 3 rabbits were fixed for 13 or 20 d in 4% paraformaldehyde, rinsed for 30 min in phosphate-buffered saline, and then homogenized. Listed are the tissue weight, size, and CFU recovered. +/– indicates presence and/or absence of growth in broth/on plate. ND, not done.
†50% of sample tested.

Main Article

1These authors contributed equally to this article.

Page created: April 18, 2019
Page updated: April 18, 2019
Page reviewed: April 18, 2019
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