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Volume 25, Number 9—September 2019
Research Letter

Fatal Case of Lassa Fever, Bangolo District, Côte d’Ivoire, 2015

Mathieu MateoComments to Author , Caroline Picard, Yahaya Sylla, Emilie Kamo, Danielle Odegue, Alexandra Journeaux, Stéphane Kouassi Kan, Marcelle Money, David N’Golo Coulibaly, Eugène Koffi, Souleymane Meite, Véronique Akran, Hervé Kadjo, Edgard Adjogoua, Solange N’Gazoa Kakou, Sylvain Baize, and Mireille Dosso
Author affiliations: Institut Pasteur, Lyon, France (M. Mateo, C. Picard, A. Journeaux, S. Baize); Centre International de Recherche en Infectiologie, Lyon (M. Mateo, C. Picard, A. Journeaux, S. Baize); Institut Pasteur de Côte d’Ivoire, Abidjan, Côte d'Ivoire (Y. Sylla, E. Kamo, D. Odegue, S.K. Kan, M. Money, D. N’Golo Coulibaly, E. Koffi, S. Meite, V. Akran, H. Kadjo, E. Adjogoua, S. N’Gazoa Kakou, S. Baize, M. Dosso)

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Figure

Analysis of LASV strains, Bangolo District, Côte d’Ivoire, 2015. A) RT-PCR analysis of human serum samples 132/16 and 001/15 by using OW and LASV RT-PCRs. Lane 1, negative control; lane 2, positive control; lane 3, 132/16; lane 4, 001/15. B) Phylogenetic analysis of LASV strains. Trees were inferred by using the PhyML Smart Model Selection (5) general time reversible plus gamma plus proportion of invariable sites model with 200 bootstrap replicates. Poorly supported branches with bootstrap value

Figure. Analysis of LASV strains, Bangolo District, Côte d’Ivoire, 2015. A) RT-PCR analysis of human serum samples 132/16 and 001/15 by using OW and LASV RT-PCRs. Lane 1, negative control; lane 2, positive control; lane 3, 132/16; lane 4, 001/15. B) Phylogenetic analysis of LASV strains. Trees were inferred by using the PhyML Smart Model Selection (5) general time-reversible plus gamma plus proportion of invariable sites model with 200 bootstrap replicates. Poorly supported branches with bootstrap values <0.50 are indicated by dotted lines. Lassa virus lineages are indicated by the Roman numerals on the right. The Bangolo-CIV-2015 strain (solid box), which was isolated in this study, appears to be related to clade IV, and the AV strain (dotted box) is related to clade V. GPC, glycoprotein complex gene; L, large RNA segment; LASV, Lassa virus; OW, Old World; RT-PCR, reverse transcription PCR.

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