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Volume 25, Number 9—September 2019
Research Letter

Characterization of Clinical Isolates of Talaromyces marneffei and Related Species, California, USA

Linlin Li, Katelyn Chen, Nirmala Dhungana, Yvonne Jang, Vishnu Chaturvedi1, and Ed Desmond2Comments to Author 
Author affiliations: California Department of Public Health, Richmond, California, USA

Main Article

Figure

Phylogenetic analysis of Talaromyces species based on concatenated nucleotide alignments of internal transcribed spacer, partial β-tubulin gene, and partial RNA polymerase II largest subunit gene regions, showing the relationship among clinical isolates from patients in California, USA (black squares), and reference Talaromyces species. The tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA software (https://www.megasoftware.net). Bootstrap support

Figure. Phylogenetic analysis of Talaromyces species based on concatenated nucleotide alignments of internal transcribed spacer, partial β-tubulin gene, and partial RNA polymerase II largest subunit gene regions, showing the relationship among clinical isolates from patients in California, USA (black squares), and reference Talaromyces species. The tree was constructed by the neighbor-joining method with 1,000 bootstrap replicates by using MEGA software (https://www.megasoftware.net). Bootstrap support values >70% are presented at the nodes. The tree was rooted with Trichocoma paradoxa CBS 788.83. GenBank accession numbers for newly generated sequences are MK601832–41 for the internal transcribed spacer, MK626499–508 for the β-tubulin gene, and MK626509–518 for the RNA polymerase II largest subunit gene. CBS, Westerdijk Fungal Biodiversity Institute; MDL, Microbial Diseases Laboratory, California Department of Public Health. Scale bar indicates estimated phylogenetic divergence.

Main Article

1Current affiliation: New York State Department of Health, Albany, New York, USA.

2Current affiliation: Hawaii State Department of Health, Pearl City, Hawaii, USA.

Page created: August 21, 2019
Page updated: August 21, 2019
Page reviewed: August 21, 2019
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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