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Volume 26, Number 1—January 2020
Research

High Pathogenicity of Nipah Virus from Pteropus lylei Fruit Bats, Cambodia

Maria Gaudino, Noémie Aurine, Claire Dumont, Julien Fouret, Marion Ferren, Cyrille Mathieu, Olivier Reynard, Viktor E. Volchkov, Catherine Legras-Lachuer, Marie-Claude Georges-Courbot, and Branka HorvatComments to Author 
Author affiliations: Centre International de Recherche en Infectiologie, CIRI, INSERM U1111, CNRS, UMR5308, Univ Lyon, University Claude Bernard Lyon 1, École Normale Supérieure de Lyon, Lyon, France (M. Gaudino, N. Aurine, C. Dumont, J. Fouret, M. Ferren, C. Mathieu, O. Reynard, V.E. Volchkov, M.-C. Georges-Courbot, B. Horvat); ViroScan 3D, Trévoux, France (J. Fouret, C. Legras-Lachuer); University Claude Bernard Lyon 1, LEM, UMR5557, CNRS, INRA, VetAgro Sup, Lyon (C. Legras-Lachuer); Unité de Biologie des Infections Virales Emergentes, Institut Pasteur, INSERM P4, Jean Mérieux, Lyon (M.-C. Georges-Courbot)

Main Article

Figure 3

Evaluation of entry of VSVΔG-RFPs (vesicular stomatitis virus in which the envelope glycoprotein G gene is replaced with the red fluorescent protein gene) pseudotyped with the surface glycoproteins of NiVs CSUR381 (Cambodia 2003 isolate), UMMC1 (NiV-Malaysia isolate), and SPB200401066 (NiV-Bangladesh isolate) in different cell types. Infections of HPMEC, NCI-H358 (human bronchioalveolar cells), PATGV1.12 (bat cells), and Vero cells were performed at a multiplicity of infection of 0.3, and the pe

Figure 3. Evaluation of entry of VSVΔG-RFPs (vesicular stomatitis virus in which the envelope glycoprotein G gene is replaced with the red fluorescent protein gene) pseudotyped with the surface glycoproteins of NiVs CSUR381 (Cambodia 2003 isolate), UMMC1 (NiV-Malaysia isolate), and SPB200401066 (NiV-Bangladesh isolate) in different cell types. Infections of HPMEC, NCI-H358 (human bronchioalveolar cells), PATGV1.12 (bat cells), and Vero cells were performed at a multiplicity of infection of 0.3, and the percentages of infected cells were evaluated 6 hours postinfection by measuring RFP by flow cytometry and normalizing values to those from Vero cells. Histograms indicate the mean of 3 independent experiments, and error bars indicate upper half of SD. HPMEC, human pulmonary microvascular endothelial cell; NiV, Nipah virus.

Main Article

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