Volume 26, Number 11—November 2020
Research
Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories
Table 3
Sterility of select agent samples after sterile filtration and methanol procedure*‡
Agent (reference) | Positive serum | Positive plasma | Positive BAL | Positive overnight culture | Positive BAL cell pellet |
---|---|---|---|---|---|
Bacillus anthracis | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Yersinia pestis | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Burkholderia mallei | 0/3 | 0/3 | 0/3 | 0/3 | NT |
Burkholderia pesudomallei | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 |
Francisella tularensis (14) | 0/6 | 0/6 | 1/6† | 1/6† | NT |
*BAL, bronchoalveolar lavage; NT, not tested. †Negative result caused by contaminated tube cap. ‡Total success rate for filtration: 97%
References
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- Centers for Disease Control and Prevention; Animal and Plant Health Inspection Service. Guidance on the Inactivation or Removal of Select Agents or Toxins for Future Use. 2018 [cited 2019 Aug 4]. https://www.selectagents.gov/irg-intro.html
- US Government Accountability Office. Actions Needed to Improve Management of DOD's Biosafety and Biosecurity Program. 2018 [cited 2019 Aug 4]. https://www.gao.gov/products/GAO-18-422
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