Figure 5. Phylogeny of Mycoplasma bovis isolates from free-ranging pronghorn (red branch), Wyoming, USA, February–April 2019. Pronghorn were found to be divergent from all bovine isolates with a deletion of adh-1 gene but are most similar to those recovered from cattle in the United States. This unrooted maximum-likelihood tree (10,000 bootstrap replicates) comprises all available nontypeable isolates and is based on 6 of 7 sequence typing loci. The health status of cattle sampled during 2011–2014 is unknown, and the absence of reported clinical signs does not necessarily equate to absence of disease. Scale bar indicates substitutions per site.