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Volume 26, Number 12—December 2020
Research Letter

High Coxiella burnetii Seroconversion Rate in Veterinary Students, the Netherlands, 2006–2010

Marit M.A. de LangeComments to Author , Wim van der Hoek, Peter M. Schneeberger, Arno Swart, Dick J.J. Heederik, Barbara Schimmer, and Inge M. Wouters
Author affiliations: National Institute for Public Health and the Environment, Bilthoven, the Netherlands (M.M.A. de Lange, W. van der Hoek, A. Swart, B. Schimmer); Jeroen Bosch Hospital, ‘s-Hertogenbosch, the Netherlands (P.M. Schneeberger); Utrecht University, Utrecht, the Netherlands (D.J.J. Heederik, I.M. Wouters)

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Abstract

We examined Coxiella burnetii seroconversion rates by measuring C. burnetii IgG among 2 cohorts of veterinary students. During follow-up of 118 seronegative veterinary students, 23 students seroconverted. Although the clinical importance of the presence of antibodies is unknown, veterinary students should be informed about the potential risks for Q fever.

Q fever is caused by the bacteria Coxiella burnetii and can manifest as acute or chronic illness. Veterinarians who care for livestock are prone to C. burnetii infection (1,2). A high seroprevalence among veterinary students has been reported (35). However, the incidence of Q fever and associated risk factors during veterinary training are still unknown. We conducted a longitudinal study at the Faculty of Veterinary Medicine of Utrecht University (FVMUU), Utrecht, the Netherlands, in which we followed incoming, seronegative veterinary students and investigated potential associated factors for seroconversion.

Veterinary students who started in 2006 or 2008 at FVMUU were invited to participate. After obtaining written informed consent, we collected blood samples, and participants completed a baseline questionnaire. From participants who began at FVMUU in 2006 (cohort 2006), <2 additional blood samples and follow-up questionnaires were obtained in 2008 and 2010. Students who started in 2008 (cohort 2008) provided 1 follow-up blood sample and 1 follow-up questionnaire in 2010.

Serum samples were tested for IgG against phase I and II of C. burnetii, using an indirect immunofluorescence assay as previously described (3). Those samples with IgG phase I or II IgG >1:32 were classified as C. burnetii seropositive. Seroconversion was defined as the change observed in a participant who was IgG seronegative at baseline and seropositive in a follow-up sample.

We determined differences in demographics and past animal exposure between seropositive and seronegative participants at baseline. Risk factors for seroconversion were estimated by using univariable logistic regression analyses through generalized estimating equations models (Appendix).

At the beginning of their veterinary training, 447 students were invited to participate in the study. Of those, 131 participated, of whom 13 (10%) were C. burnetii IgG seropositive at baseline. Students who were seropositive at baseline were more likely to have lived on a farm and to have had contact with cattle and poultry (Appendix Table 1).

Figure

Follow-up timeline illustrating number and percentages of seronegative participants at baseline, per follow-up moment, in study of Coxiella burnetii seroconversion rate in veterinary students, the Netherlands, 2006–2010. The 17 seropositive students in 2010 include the 11 students who already seroconverted during 2006–2008 and were censored from risk factor analysis in 2010.

Figure. Follow-up timeline illustrating number and percentages of seronegative participants at baseline, per follow-up moment, in study of Coxiella burnetiiseroconversion rate in veterinary students, the Netherlands, 2006–2010. The 17...

Of the 118 participants seronegative at baseline, 78 started their training in 2006 and 40 in 2008 (Figure). Of those students, 23 seroconverted during the follow-up period of 362 person-years, translating to an incidence of 0.06/person-year. Of the 17 seroconversions in cohort 2006, 11 occurred between baseline and the first follow-up, and 4 occurred between the first and second follow-up (Appendix Table 2). None of the seroconverted participants reported a diagnosis of acute Q fever from a general practitioner or medical specialist, suggesting all cases were mild or asymptomatic. In addition, no participants had serologic indication of a chronic infection. Of the 20 investigated characteristics, “living on a sheep or goat farm,” “having contact with sheep outside [veterinary] training,” and “working with hay, straw, silage grass, or animal feed” outside FVMUU increased the odds of seroconversion (Table).

We were not able to identify education-related potential risk factors, such as courses taken, for 2 reasons. First, the curriculum changed during our study, so participants from the 2006 and 2008 cohorts took different courses, causing low power in the analysis. Second, within each cohort, little variation occurred in courses taken. Another limitation of this study is our assumption of a constant risk for C. burnetii exposure during the study period. Students seem to have been at higher risk for infection in the first 2 study years, although we cannot draw definite conclusions from this small group of students.

Identified risk factors for seroconversion were not education-related. Proximity to (aborting) small ruminants, such as goats and sheep, was a risk factor in an outbreak in the Netherlands (6). Veterinary students have a high prevalence of animal contacts outside their education (7). In addition, contact with hay, straw, silage grass, or animal feed, is a known risk factor for human Q fever (8). A major outbreak of acute Q fever occurred in the Netherlands during 2007–2010 (9), and some students might have contracted the infection then, although increased seroprevalence of Q fever in veterinary students before that outbreak has been reported (3).

In conclusion, we found a considerable C. burnetii seroconversion rate among veterinary students. Although the clinical importance of the presence of antibodies is unknown, students should be advised at the beginning of their education about potential risks and instructed to seek care if they experience symptoms of acute or chronic Q fever infection.

Dr. de Lange is an epidemiologist at the National Institute for Public Health and the Environment. She conducted her PhD research on Q fever. Her other research interests include respiratory infections, such as influenza and respiratory syncytial virus.

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Acknowledgment

We would like to express our gratitude to all participants of the study. Many thanks to coworkers at the Institute for Risk Assessment Sciences involved in recruitment of the participants and serum sample collection (Lot Bannink, Manon Bogaerts, Isabella van Schothorst, Esmeralda Krop, Siegfried de Wind, Jack Spithoven, Marieke Oldenwening, Lidwien Smit, Haitske Gravenland, and Bernadette Aalders). We would like to thank the laboratory technicians in the Serology Unit of the Department of Medical Microbiology and Infection Control at Jeroen Bosch Hospital for their work in analyzing serum samples. We would also like to thank Roel Coutinho of the Julius Center University Medical Center Utrecht for reviewing the manuscript.

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References

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  7. Samadi  S, Spithoven  J, Jamshidifard  AR, Berends  BR, Lipman  L, Heederik  DJ, et al. Allergy among veterinary medicine students in The Netherlands. Occup Environ Med. 2012;69:4855. DOIPubMed
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  9. van der Hoek  W, Morroy  G, Renders  NH, Wever  PC, Hermans  MH, Leenders  AC, et al. Epidemic Q fever in humans in the Netherlands. Adv Exp Med Biol. 2012;984:32964. DOIPubMed

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Cite This Article

DOI: 10.3201/eid2612.200063

Original Publication Date: November 08, 2020

Table of Contents – Volume 26, Number 12—December 2020

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Address for Correspondence: Marit M.A. de Lange, National Institute for Public Health and the Environment, Centre for Infectious Disease Control Netherlands, Antonie van Leeuwenhoeklaan 9, 3721 MA, Bilthoven, the Netherlands

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