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Volume 26, Number 12—December 2020
Research Letter

Autochthonous Ratborne Seoul Virus Infection in Woman with Acute Kidney Injury

Jörg Hofmann1Comments to Author , Elisa Heuser1, Sabrina Weiss2, Beate Tenner, Konrad Schoppmeyer, Jutta Esser, Christiane Klier, Stephan Drewes, Rainer G. Ulrich, and Detlev H. Kruger
Author affiliations: Charité–Universitätsmedizin Berlin, Berlin, Germany (J. Hofmann, S. Weiss, B. Tenner, D.H. Kruger); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (E. Heuser, S. Drewes, R.G. Ulrich); Euregio-Klinik, Medizinische Klinik II, Nordhorn, Germany (K. Schoppmeyer); Laborarztpraxis Osnabrück, Georgsmarienhütte, Germany (J. Esser); German Center for Infection Research, Partner Site Hamburg-Lübeck-Borstel-Insel Riems, Germany (E. Heuser, R.G. Ulrich); Public Health Agency of Lower Saxony, Hannover, Germany (C. Klier)

Main Article

Figure

Molecular phylogenetic analysis of the amplified large (L) and small (S) segment regions of human and rat origin from Nordhorn/Germany (strains Nordhorn GER Hu and Nordhorn GER Rn, designated in bold). The consensus tree is based on a 412-nt region of the L segment (A) and a 673-nt region of the S segment (B). Alignments were constructed with Bioedit software package version7.2.5) (https://bioedit.software.informer.com) using the Clustal W Multiple Alignment algorithm. The best fitting substitution model was determined with jModeltest version 2.1.10 (https://github.com/ddarriba/jmodeltest2). Trees were reconstructed with MrBayes version 3.2.6 (http://www.mrbayes.net) and FasttreeMP version 2.1.10 (http://microbesonline.org/fasttree) executed on the CIPRES portal (https://www.phylo.org) according to maximum-likelihood and Markov chain Monte Carlo algorithms. The consensus tree is based on Bayesian analyses with 2 × 106 generations, a burn-in phase of 25%, and the Hasegawa-Kishono-Yano substitution model with gamma distribution. Bootstrap values were transferred to the Bayesian tree behind posterior probabilities only if they were >50% and if branches of both trees were consistent. Hantaan virus was used as outgroup. The L and S segment sequences were deposited in GenBank under accession nos. MT123530–33. At the end of the strain names the country of origin is given: BEL, Belgium; CHN, China; FRA, France; GBR, Great Britain; GER, Germany; INA, Indonesia; KOR, South Korea; NED, the Netherlands; USA, United States. Scale bars indicate nucleotide substitutions per site.

Figure. Molecular phylogenetic analysis of the amplified large (L) and small (S) segment regions of human and rat origin from Nordhorn/Germany (strains Nordhorn GER Hu and Nordhorn GER Rn, designated in bold). The consensus tree is based on a 412-nt region of the L segment (A) and a 673-nt region of the S segment (B). Alignments were constructed with Bioedit software package version7.2.5) (https://bioedit.software.informer.com) using the Clustal W Multiple Alignment algorithm. The best fitting substitution model was determined with jModeltest version 2.1.10 (https://github.com/ddarriba/jmodeltest2). Trees were reconstructed with MrBayes version 3.2.6 (http://www.mrbayes.net) and FasttreeMP version 2.1.10 (http://microbesonline.org/fasttree) executed on the CIPRES portal (https://www.phylo.org) according to maximum-likelihood and Markov chain Monte Carlo algorithms. The consensus tree is based on Bayesian analyses with 2 × 106 generations, a burn-in phase of 25%, and the Hasegawa-Kishono-Yano substitution model with gamma distribution. Bootstrap values were transferred to the Bayesian tree behind posterior probabilities only if they were >50% and if branches of both trees were consistent. Hantaan virus was used as outgroup. The L and S segment sequences were deposited in GenBank under accession nos. MT123530–33. At the end of the strain names the country of origin is given: BEL, Belgium; CHN, China; FRA, France; GBR, Great Britain; GER, Germany; INA, Indonesia; KOR, South Korea; NED, the Netherlands; USA, United States. Scale bars indicate nucleotide substitutions per site.

Main Article

1These authors contributed equally to this article.

2Current affiliation: Robert Koch Institute, Berlin, Germany.

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