Volume 26, Number 12—December 2020
Research
Trends in Population Dynamics of Escherichia coli Sequence Type 131, Calgary, Alberta, Canada, 2006–20161
Table 4
Virulence factors associated with Escherichia coli sequence type 131 clades, Calgary, Alberta, Canada, 2006, 2012, and 2016*
| Factor | Clade |
||||||
|---|---|---|---|---|---|---|---|
| A, n = 34 | B, n = 32 | C0, n = 5 | C1-nonM27, n = 121 | C1-M27, n = 13 | C2, n = 139 | All, n = 344 | |
| Adhesion gene | |||||||
| papA | 34 (100) | 28 (88) | 5 (100) | 121 (100) | 13 (100) | 139 (100) | 340 (99) |
| papB | 1 (3)a | 11 (34) | 0 | 35 (29)a | 1 (8) | 90 (65)b | 138 (40) |
| papC | 1 (3)a | 13 (41) | 0 | 31 (26)a | 2 (16) | 89 (64)b | 141 (41) |
| papD | 1 (3)a | 13 (41) | 0 | 36 (30)a | 1 (8) | 89 (64)b | 140 (41) |
| papE | 1 (3) | 11 (34) | 0 | 0 | 0 | 9 (6) | 21 (6) |
| papF | 2 (6)a | 12 (38) | 0 | 34 (28)a | 1 (8) | 90 (65)b | 139 (40) |
| papG | 1 (3) | 14 (44) | 0 | 34 (28) | 1 (8) | 91 (6) | 141 (41) |
| papH | 1 (3) | 9 (28) | 0 | 33 (27) | 0 | 41 (29) | 84 (24) |
| papI | 32 (94) | 24 (75)a | 5 (100) | 116 (96) | 12 (92) | 137 (99)b | 326 (95) |
| papJ | 1 (3)a | 11 (34) | 0 | 34 (28)a | 1 (8) | 90 (65)b | 137 (40) |
| papK | 1 (3)a | 11 (34) | 0 | 34 (28)a | 1 (8) | 90 (65)b | 137 (40) |
| papX | 32 (94) | 21 (66)a | 5 (100) | 100 (83) | 10 (77) | 134 (96)b | 302 (88) |
| Iha | 33 (97) | 16 (50)a | 5 (100) | 111 (92) | 13 (100) | 137 (99)b | 314 (91) |
| fimH | 34 (100) | 32 (100) | 5 (100) | 121 (100) | 13 (100) | 139 (100) | 344 (100) |
| Tsh | 0 | 2 (6) | 0 | 0 | 0 | 0 | 2 (0.6) |
| Hra | 1 (1) | 0 | 1 (25) | 1 (1) | 0 | 8 (6) | 11 (3) |
| afaABCD | 18 (53)a | 18 (56)a | 1 (25) | 4 (3)b | 0 | 28 (20)b | 69 (20) |
| draABCDP |
18 (53)a |
18 (56)a |
1 (25) |
4 (3)b |
0 |
28 (20)b |
69 (20) |
| Toxin gene | |||||||
| hlyA | 1 (1) | 11 (34) | 0 | 27 (22) | 0 | 50 (36) | 89 (26) |
| Sat | 30 (88) | 21 (66)a | 5 (100) | 112 (93) | 13 (100) | 136 (98)b | 317 (92) |
| Vat | 34 (100)a | 7 (22)b | 0 | 1 (1)b | 0b | 0b | 42 (1) |
| astA | 1 (3) | 1 (3) | 0 | 0 | 0 | 6 (4) | 8 (2) |
| cnf1 |
1 (3) |
9 (28) |
0 |
29 (24) |
0 |
52 (37) |
91 (26) |
| Siderophore gene | |||||||
| iroN | 0 | 9 (28) | 0 | 1 (1) | 0 | 9 (6) | 19 (6) |
| fyuA | 34 (100) | 32 (100) | 5 (100) | 121 (100) | 13 (100) | 139 (100) | 344 (100) |
| ireA | 0 | 0 | 0 | 0 | 0 | 11 (8) | 11 (3) |
| iutA |
29 (85) |
27 (84) |
5 (100) |
112 (93) |
12 (92) |
137 (99) |
322 (94) |
| Capsular antigen gene | |||||||
| kpsM II | 0 | 14 (44) | 0 | 0 | 0 | 2 (1) | 16 (5) |
| kpsMT III |
0a |
13 (41) |
5 (100) |
71 (59)a,b |
13 (100)b |
37 (26)c |
139 (40) |
| Miscellaneous gene | |||||||
| Usp | 33 (97) | 32 (100) | 5 (100) | 121 (100) | 12 (92) | 137 (99) | 340 (99) |
| traT | 28 (82)a | 13 (41)b | 0 | 20 (17)b | 0b | 28 (20)b | 89 (26) |
| ompT | 0 | 4 (13) | 0 | 2 (2) | 0 | 1 (1) | 7 (2) |
| Iss | 9 (26)a | 31 (97) | 5 (100) | 118 (98)b | 13 (100) | 139 (100)b | 315 (92) |
| malX | 34 (100) | 32 (100) | 5 (100) | 118 (98) | 13 (100) | 138 (99) | 340 (99) |
| cdtB | 0 | 2 (6) | 0 | 0 | 0 | 0 | 2 (0.6) |
| cvaC | 0 | 4 (13) | 0 | 0 | 9 (69%) | 10 (7%) | 14 (6) |
| ibeABC |
0 |
14 (44)a |
0 |
0 |
0 |
0b |
14 (6) |
| Virulence score, median (range)† | 11 (7–15) | 10 (6–14) | 11 (9–16) | 10 (6–15) | 10 (9–14) | 11 (7–15) | |
*Values are no. (%) unless indicated otherwise. Rates followed by different superscript letters indicate significant differences between clades at the 5% level (adjusted for multiple comparisons). afa, afimbrial adhesin; astA, enteroaggregative E. coli toxin; cdtB, cytolethal distending toxin B; cnf1, cytotoxic necrotizing factor; cvaC, factor facilitating colonization; dra, Dr binding adhesins; fimH, type-1 fimbriae; fyuA, yersiniabactin (siderophore) receptor; hlyA, α-hemolysin; hra, heat-resistant agglutinin; ibeABC, invasion of brain endothelium; iha, iron-regulated adhesin; ireA, iron-regulated element (catecholate siderophore); iroN, salmochelin (siderophore) receptor; iss, increased serum survival; iutA, aerobactin (siderophore) receptor; kpsM II, group II capsule variants synthesis; kpsM III, group III capsule variants synthesis; malX, pathogenicity island marker; ompT, outer membrane protein T; papA, P fimbriae; papBCDEFGHIJKX, genes of P fimbriae operon; sat, secreted autotransporter toxin; traT, complement inhibition protein; tsh, temperature-sensitive hemagglutinin; usp, uropathogenic-specific protein; vat, vacuolating autotransporter. toxin. †The virulence gene score was the number of virulence operons detected.
1Accepted as an oral presentation for the 30th European Congress of Clinical Microbiology and Infectious Diseases, Paris, France, April 18–21, 2020.
2These authors contributed equally to this article.