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Volume 26, Number 12—December 2020
Research

Equine-Like H3 Avian Influenza Viruses in Wild Birds, Chile

Nicolas Bravo-Vasquez1, Jiangwei Yao1, Pedro Jimenez-Bluhm1Comments to Author , Victoria Meliopoulos, Pamela Freiden, Bridgett Sharp, Leonardo Estrada, Amy Davis, Sean Cherry, Brandi Livingston, Angela Danner, Stacey Schultz-Cherry, and Christopher Hamilton-WestComments to Author 
Author affiliations: University of Texas Health Science Center, Houston, Texas, USA (N. Bravo-Vasquez); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Yao); University of Chile, Santiago, Chile (P. Jimenez-Bluhm, C. Hamilton-West); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (V. Meliopoulos, P. Freiden, B. Sharp, L. Estrada, A. Davis, S. Cherry, B. Livingston, A. Danner, S. Schultz-Cherry)

Main Article

Figure 5

Replicative capacity of H3Nx influenza viruses in vitro. A, B) To evaluate the replication of H3N8 viruses in vitro, MDCK cells (A) and human lung A549 cells (B) were infected at a multiplicity of infection of 0.01, and cell culture supernatants were collected at 6, 16, 24, 48, and 72 hours postinfection. Viral titers were determined by TCID50 analysis in triplicate. Values are mean titers of 3 replicates, and error bars indicate SEMs. Differences were considered significant at p<0.05 (*). C) Cumulative shedding for each cell line and each viral strain shown. TCID50, 50% median tissue culture infectious dose.

Figure 5. Replicative capacity of H3Nx influenza viruses in vitro. A, B) To evaluate the replication of H3N8 viruses in vitro, MDCK cells (A) and human lung A549 cells (B) were infected at a multiplicity of infection of 0.01, and cell culture supernatants were collected at 6, 16, 24, 48, and 72 hours postinfection. Viral titers were determined by TCID50 analysis in triplicate. Values are mean titers of 3 replicates, and error bars indicate SEMs. Differences were considered significant at p<0.05 (*). C) Cumulative shedding for each cell line and each viral strain shown. TCID50, 50% median tissue culture infectious dose.

Main Article

1These authors contributed equally to this article.

Page created: July 27, 2020
Page updated: November 19, 2020
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