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Volume 26, Number 12—December 2020
Research Letter

SARS-CoV-2 Natural Transmission from Human to Cat, Belgium, March 2020

Mutien Garigliany, Anne-Sophie Van Laere, Cécile Clercx, Didier Giet, Nicolas Escriou, Christèle Huon, Sylvie van der Werf, Marc Eloit, and Daniel DesmechtComments to Author 

Author affiliations: University of Liège, Liège, Belgium (M. Garigliany, A.-S. Van Laere, C. Clercx, D. Giet, D. Desmecht); Institut Pasteur, Paris, France (N. Escriou, C. Huon, S. van der Werf, M. Eloit); Alfort National Veterinary School, Maisons Alfort, France (M. Eloit)

Main Article

Table

Severe acute respiratory syndrome coronavirus 2 genome loads measured by qRT-PCR in a series of consecutive swab samples from cat, Belgium, March 2020*

Date Oropharyngeal swab samples
  Vomitus
  Feces
β-actin gene N gene β−actin gene N gene β-actin gene N gene
11 NS NS   26.95 23.5 ± 0.1   35.5 33.3 ± 0.2
12 33.4 38.2 ± 0.5   NS NS   32.9 34.8 ± 0.0
13 37.8 37.9 ± 0   ND 34.9 ± 0.1   34.4 37,6 ± 0,1
14 25.1 39.3 ± 0.1   NS NS   30.7 35.1 ± 0.1
15 35.9 35.7 ± 0.1   NS NS   27.8 33.2 ± 0.1
16 38.2 Negative   NS NS   26.0 35.1 ± 0
17 27.1 38.2 ± 0   NS NS   28.7 Negative
18 26.7 Negative   NS NS   36.1 37.9 ± 0
19 NS NS   NS NS   27.9 39.0 ± 0
20 NS NS   NS NS   30.1 Negative
21 NS NS   29.9 33.8 ± 0.1   32.8 Negative
22 37.9 Negative   NS NS   31.7 Negative
23 NS NS   NS NS   33.8 Negative
25
NS
NS
34.9
Negative
35.0
Negative
*Numbers reported are defined as the number of cycles required for the real-time PCR assay fluorescent signal curve to intersect with a threshold line that exceeds background level (mean ± SD). It is a relative measure of the concentration of the genomic target in the qRT-PCR reaction (the severe acute respiratory syndrome coronavirus 2 N gene or cat β-actin gene); values >40 are considered negative. All samples with qRT-PCR values <40 were analyzed further by a standard gel RT-PCR targeting the coding sequence of the virus spike protein gene followed by Sanger sequencing of the correctly sized amplicon retrieved (»370 bp). Only samples positive for all 3 tests were defined as positive, which was the case for all samples with a value <40 for the N gene aggregated in this table. NS, no sample available; negative, RT-qPCR and/or gel PCR and/or sequencing test failed; qRT-PCR, quantitative reverse transcription PCR.
 

Main Article

Page created: August 15, 2020
Page updated: August 17, 2020
Page reviewed: August 17, 2020
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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