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Volume 26, Number 12—December 2020
Dispatch

Detection and Characterization of Bat Sarbecovirus Phylogenetically Related to SARS-CoV-2, Japan

Shin Murakami1Comments to Author , Tomoya Kitamura1, Jin Suzuki, Ryouta Sato, Toshiki Aoi, Marina Fujii, Hiromichi Matsugo, Haruhiko Kamiki, Hiroho Ishida, Akiko Takenaka-Uema, Masayuki Shimojima, and Taisuke HorimotoComments to Author 
Author affiliations: The University of Tokyo, Tokyo, Japan (S. Murakami, T. Kitamura, M. Fujii, H. Matsugo, H. Kamiki, H. Ishida, A. Takenaka-Uema, T. Horimoto); Yamaguchi University, Yamaguchi, Japan (J. Suzuki); Iwate University, Iwate, Japan (R. Sato, T. Aoi); National Institute of Infectious Diseases, Tokyo, Japan (M. Shimojima)

Main Article

Figure 2

Infectivity titers of sarbecoviruses from bats and humans used to investigate bat sarbecovirus Rc-o319, which is genetically related to human SARS-CoV-2, Japan. Cells expressing each host-origin angiotensin-converting enzyme 2 were inoculated with VSV pseudotyped with spike proteins of Rc-o319 (A), SARS-CoV (B), SARS-CoV-2 (C), or glycoprotein of VSV (D). At 20 hour postinfection, GFP-positive cells were counted and the infectivity titers were calculated. Error bars indicate SDs from 3 independent experiments. CoV, coronavirus; GFP, green fluorescent protein; Rc/Rf, chimera of Rhinolophus cornutus and R. ferrumequinum; SARS, severe acute respiratory syndrome; VSV, vesicular stomatitis virus.

Figure 2. Infectivity titers of sarbecoviruses from bats and humans used to investigate bat sarbecovirus Rc-o319, which is genetically related to human SARS-CoV-2, Japan. Cells expressing each host-origin angiotensin-converting enzyme 2 were inoculated with VSV pseudotyped with spike proteins of Rc-o319 (A), SARS-CoV (B), SARS-CoV-2 (C), or glycoprotein of VSV (D). At 20 hour postinfection, GFP-positive cells were counted and the infectivity titers were calculated. Error bars indicate SDs from 3 independent experiments. CoV, coronavirus; GFP, green fluorescent protein; Rc/Rf, chimera of Rhinolophus cornutus and R. ferrumequinum; SARS, severe acute respiratory syndrome; VSV, vesicular stomatitis virus.

Main Article

1These authors contributed equally to this article.

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