TY - JOUR AU - Van Driessche, Laura AU - Vanneste, Kevin AU - Bogaerts, Bert AU - De Keersmaecker, Sigrid C.J. AU - Roosens, Nancy AU - Haesebrouck, Freddy AU - De Cremer, Lieze AU - Deprez, Piet AU - Pardon, Bart AU - Boyen, Filip T1 - Isolation of Drug-Resistant Gallibacterium anatis from Calves with Unresponsive Bronchopneumonia, Belgium T2 - Emerging Infectious Disease journal PY - 2020 VL - 26 IS - 4 SP - 721 SN - 1080-6059 AB - Gallibacterium anatis is an opportunistic pathogen, previously associated with deaths in poultry, domestic birds, and occasionally humans. We obtained G. anatis isolates from bronchoalveolar lavage samples of 10 calves with bronchopneumonia unresponsive to antimicrobial therapy. Collected isolates were multidrug-resistant to extensively drug-resistant, exhibiting resistance against 5–7 classes of antimicrobial drugs. Whole-genome sequencing revealed 24 different antimicrobial-resistance determinants, including genes not previously described in the Gallibacterium genus or even the Pasteurellaceae family, such as aadA23, blaCARB-8, tet(Y), and qnrD1. Some resistance genes were closely linked in resistance gene cassettes with either transposases in close proximity or situated on putative mobile elements or predicted plasmids. Single-nucleotide polymorphism genotyping revealed large genetic variation between the G. anatis isolates, including isolates retrieved from the same farm. G. anatis might play a hitherto unrecognized role as a respiratory pathogen and resistance gene reservoir in cattle and has unknown zoonotic potential. KW - Gallibacterium anatis KW - infectious bronchopneumonia KW - cattle KW - multiresistant KW - whole genome sequencing KW - therapy failure KW - MALDI-TOF MS KW - zoonoses KW - respiratory diseases KW - antimicrobial resistance KW - extensively drug-resistant KW - multidrug-resistant KW - whole-genome sequencing KW - MALDI-TOF KW - mass spectrometry KW - Belgium DO - 10.3201/eid2604.190962 UR - https://wwwnc.cdc.gov/eid/article/26/4/19-0962_article ER - End of Reference