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Volume 26, Number 5—May 2020
Research Letter

Serologic Detection of Middle East Respiratory Syndrome Coronavirus Functional Antibodies

Nisreen M.A. Okba, Ivy Widjaja, Wentao Li, Corine H. GeurtsvanKessel, Elmoubasher A.B.A. Farag, Mohammed Al-Hajri, Wan Beom Park, Myoung-don Oh, Chantal B.E.M. Reusken, Marion P.G. Koopmans, Berend-Jan BoschComments to Author , and Bart L. HaagmansComments to Author 
Author affiliations: Erasmus Medical Center, Rotterdam, the Netherlands (N.M.A. Okba, C.H. GeurtsvanKessel, C.B.E.M. Reusken, M.P.G. Koopmans, B.L. Haagmans); Utrecht University, Utrecht, the Netherlands (I. Widjaja, W. Li, B.-J. Bosch); Ministry of Public Health, Doha, Qatar (E.A.B.A. Farag, M. Al-Hajri); Seoul National University College of Medicine, Seoul, South Korea (W.B. Park, M.-d. Oh); Center for Infectious Disease Control, Bilthoven, the Netherlands (C.B.E.M. Reusken)

Main Article

Figure

MERS-CoV–specific RBI and HI assays for MERS-CoV human diagnostics. A) Validation of the specificity of the RBI assay for the detection of MERS-CoV–specific antibodies in humans. Red dots indicate severe illness. Green dots indicate mild illness. B) Correlation between neutralizing and RBI antibody responses after MERS-CoV infection. C) Hemagglutination of turkey erythrocytes using S1A-nanoparticles. S1A-, S1B-, or empty self-assembling lumazine synthase nanoparticles were serially diluted and t

Figure. MERS-CoV–specific RBI and HI assays for MERS-CoV human diagnostics. A) Validation of the specificity of the RBI assay for the detection of MERS-CoV–specific antibodies in humans. Red dots indicate severe illness. Green dots indicate mild illness. B) Correlation between neutralizing and RBI antibody responses after MERS-CoV infection. C) Hemagglutination of turkey erythrocytes using S1A-nanoparticles. S1A-, S1B-, or empty self-assembling lumazine synthase nanoparticles were serially diluted and tested for the ablity to agglutinate turkey RBCs. D) Specificity of the HI assay for the detection of MERS-CoV S1A–directed antibodies. Rabbit anti-S1A, anti S1B, or anti-S1 serum samples were serially diluted and tested for the ability to block S1A-nanoparticles–induced hemagglutination of turkey RBCs. E) Validation of HI assay for the detection of MERS-CoV–specific antibodies in humans. F) Scatter plot correlating PRNT90 neutralization titers and HI titers after MERS-CoV infection. CoV, human coronavirus; HI, hemagglutination inhibition; MERS-CoV, Middle East respiratory syndrome coronavirus; PRNT90, 90% reduction in plaque reduction neutralization test; RBI, receptor-binding inhibition.

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Page created: April 16, 2020
Page updated: April 16, 2020
Page reviewed: April 16, 2020
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