Population Genomic Structure and Recent Evolution of Plasmodium knowlesi, Peninsular Malaysia
Suzanne E. Hocking, Paul C.S. Divis, Khamisah A. Kadir, Balbir Singh, and David J. Conway
Author affiliations: London School of Hygiene and Tropical Medicine Department of Infection Biology, London, UK (S.E. Hocking, D.J. Conway); Universiti Sarawak Malaysia Malaria Research Centre, Kota Samarahan, Malaysia (P.C.S. Divis, K.A. Kadir, B. Singh, D.J. Conway)
Figure 1. Locations of hospitals in peninsular Malaysia from which clinical Plasmodium knowlesi infections were sampled and sequenced in the states of Perak (Taiping and Sungai Siput), Kelantan (Gua Musang), and Pahang (Kuala Lipis and Temerloh). Of 56 infection samples processed through leukocyte depletion and subsequent DNA extraction, 32 had sufficient quantity and purity of P. knowlesi DNA for Illumina sequencing (https://www.illumina.com), of which 28 yielded high coverage genomewide sequence for population genomic analysis (sample and sequencing details listed in Appendix 1 Table).
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