Volume 26, Number 8—August 2020
CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands
|Primer or probe||DNA sequence||Position*||Description|
||5′-CGG CGG GTT AAT CAA AAT AGA A-3′
||8907–8928 (flanking pol)
||Forward primer conventional PCR
||5′-GCG GAG AAC CCC ATT TAT TAA TAA G-3′
||11334–11310 (flanking pol)
||Reverse primer conventional PCR
||5′-ATG TWG GTA RAC AAT TTC ATG TAG AAG-3′
||10919–10945 (within pol)
||5′-TCA TCA AGA CTA TTA ATA ACD GTN ACA ACA-3′
||11111–11082 (within pol)
||Reverse primer real-time PCR and typing PCR
||FAM-5-ACC AGC MGC CAT TCT ACT ACG AGH AC-3-BHQ1
||11079–11054 (within pol)
||Probe real-time PCR
|JP1crasF||5′-TAA AAC TAC WAT TTA TAG AGT TAA TAA AGA TGC STT TAG T-3′||10023–10062 (within pol)||Forward primer typing|
*The sequence position was determined against a crAssphage sequence from GenBank (accession no. NC_024711).
†Liang et al. (30).
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Page updated: July 17, 2020
Page reviewed: July 17, 2020
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