Volume 26, Number 8—August 2020
Synopsis
US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2
Xiaoyan Lu, Lijuan Wang, Senthilkumar K. Sakthivel, Brett Whitaker, Janna Murray, Shifaq Kamili, Brian Lynch, Lakshmi Malapati, Stephen A. Burke, Jennifer Harcourt, Azaibi Tamin, Natalie J. Thornburg, Julie M. Villanueva, and Stephen Lindstrom
Table 8
Test results for 2,923 human specimens determined by the US CDC real-time RT-PCR panel for detection of SARS-CoV-2*
| Specimens | Specimens for initial laboratory diagnosis, no. (%) |
Serial follow-up specimens from laboratory-
confirmed positive cases, no. (%) |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Positive | Negative | Inconclusive | Total | Positive | Negative | Inconclusive | Total | ||
| Upper respiratory tract | |||||||||
| NP swab | 42 (3.85) | 1,048 (96.06) | 1 (0.09) | 1,091 (100) | 60 (46.51) | 50 (38.76) | 19 (14.73) | 129 (100) | |
| OP swab | 33 (3.09) | 1,035 (96.91) | 0 | 1,068 (100) | 42 (30.00) | 86 (61.43) | 12 (8.57) | 140 (100) | |
| Nasal swab/wash |
0 |
7 (100) |
0 |
7 (100) |
0 |
0 |
0 |
0 |
|
| Lower respiratory tract | |||||||||
| Sputum | 5 (2.79) | 174 (97.21) | 0 | 179 (100) | 13 (72.22) | 3 (16.67) | 2 (11.11) | 18 (100) | |
| BAL | 1 (50) | 1 (50) | 0 | 2 (100) | 0 | 0 | 0 | 0 | |
| Bronchial wash | 0 | 1 (100) | 0 | 1 (100) | 0 | 0 | 0 | 0 | |
| Tissue, lung | 0 | 2 (100) | 0 | 2 (100) | 0 | 0 | 0 | 0 | |
| Tracheal aspirate |
0 |
0 |
0 |
0 |
1 (100) |
0 |
0 |
1 (100) |
|
| Other | |||||||||
| Serum | 0 | 74 (100) | 0 | 74 (100) | 4 (4.88) | 76 (92.68) | 2 (2.44) | 82 (100) | |
| Stool | 0 | 0 | 0 | 0 | 22 (40.74) | 28 (51.85) | 4 (7.41) | 54 (100) | |
| Urine | 0 | 10 (100) | 0 | 10 (100) | 0 | 62 (100) | 0 | 62 (100) | |
| Pleural fluid | 0 | 1 (100) | 0 | 1 (100) | 0 | 0 | 0 | 0 | |
| CSF |
0 |
2 (100) |
0 |
2 (100) |
0 |
0 |
0 |
0 |
|
| Total | 81 (3.32) | 2,355 (96.64) | 1 (0.04) | 2,437 (100) | 142 (29.22) | 305 (62.76) | 39 (8.02) | 486 (100) | |
*BAL, bronchoalveolar lavage; CDC, Centers for Disease Control and Prevention; CSF, cerebrospinal fluid; NP, nasopharyngeal; OP, oropharyngeal; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory coronavirus 2.
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Page updated: July 17, 2020
Page reviewed: July 17, 2020
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