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Volume 26, Number 9—September 2020
Dispatch

Identification of Streptococcus suis Meningitis by Direct Triplex Real-Time PCR, Burkina Faso

Mahamoudou OuattaraComments to Author , Mamadou Tamboura, Dinanibe Kambire, Mahamoudou Sanou, Kalifa Ouattara, Malika Congo, Adama Kaboré, Soufiane Sanou, Elie Kabré, Sable Sharpley, Theresa Tran, Stephanie Schwartz, Soumeya Ouangraoua, Abdoul-salam Ouedraogo, Lassana Sangaré, Rasmata Ouedraogo-Traore, Cynthia G. Whitney, and Bernard Beall
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (M. Ouattara, S. Sharpley, T. Tran, S. Schwartz, C.G. Whitney, B. Beall); Centre Hospitalier Universitaire Pédiatrique Charles de Gaulle, Ouagadougou, Burkina Faso (M. Tamboura, D. Kambire, M. Sanou, R. Ouedraogo-Traore); Centre Hospitalier Universitaire Yalgado Ouédraogo, Ouagadougou (K. Ouattara, M. Congo, L. Sangaré); Centre Hospitalier Universitaire Sanon-Sourô, Bobo-Dioulasso, Burkina Faso (A. Kaboré, S. Sanou, A-s, Ouedraogo); Centre-Muraz, Bobo-Dioulasso (E. Kabré, S. Ouangraoua)

Main Article

Table 1

Sequences and concentrations of primers and probes used to detect Streptococcus suis, S. agalactiae, and S. pyogenes, Burkina Faso*

Target gene Forward primer, 5¢ ® 3¢ Reverse primer, 5¢ ® 3¢ Probe, 5¢ ® 3¢ Concentration of oligo per reaction, nM†
cfb (7), GBS GGGAACAGATTATGAAAAACCG AAGGCTTCTACACGACTACCAA AGACTTCATTGCGTGCCAACCCTGAGAC
5¢-HEX; 3¢-BHQ1 200/200/200
fbpS (8), S. suis TCCRATRCTGCTCTGCCATT TGATAGTAGAAGTCCAGCARACT AATAGCCC”T”GAAAAMCAGCCACWYTTTGARA
5¢-FAM; 3¢-SpC6; “T” = BHQ1 200/200/100
spy (9), GAS GCACTCGCTACTATTTCTTACCTCAA GTCACAATGTCTTGGAAACCAGTAAT CCGCAAC”T”CATCAAGGATTTCGTTACCA
5¢-Cy-5; 3¢- SpC6; “T” = BHQ2 300/300/100
RNaseP (10) AGATTTGGACCTGCGAGCG GAGCGGCTGTCCCACAAGT TTCTGACCTGAAGGCTCTGCGCG
5¢-FAM; 3¢-BHQ1 400/400/100

*GAS, group A Streptococcus (Streptococcus pyogenes); GBS, group A Streptococcus (S. agalactiae); RNaseP, human ribonuclease P.
†Concentration of forward primer/reverse primer/probe.

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References
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CrossRef reports the first page should be "1", not "e45". (Ref. 1 "Goyette-Desjardins, Auger, Xu, Segura, Gottschalk, 2014")

eXtyles has not updated the authors because of a name mismatch. Please compare "Mai" with "Thi Hoang Mai", "Hoa" with "Thi Hoa", "Nga" with "Vu Thieu Nga", "Linh" with "Dieu Linh", "Chau" with "Thi Hong Chau", and "Sinh" with "Xuan Sinh". The CrossRef authors are Thi Hoang Mai N, Thi Hoa N, Vu Thieu Nga T, Dieu Linh L, Thi Hong Chau T, Xuan Sinh D, et al.. (Ref. 5 "Mai, Hoa, Nga, Linh, Chau, Sinh, et al., 2008")

eXtyles has not updated the article title because Medline's is slightly different and Title Case. The Medline article title is Nationwide Trends in Bacterial Meningitis before the Introduction of 13-Valent Pneumococcal Conjugate Vaccine-Burkina Faso, 2011-2013.. (Ref. 6 "Kambiré, Soeters, Ouédraogo-Traoré, Medah, Sangare, Yaméogo, et al., 2016")

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