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Volume 27, Number 1—January 2021
Research

Performance of Nucleic Acid Amplification Tests for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Prospectively Pooled Specimens

Hannah Wang, Catherine A. Hogan, Jacob A. Miller, Malaya K. Sahoo, ChunHong Huang, Kenji O. Mfuh, Mamdouh Sibai, James Zehnder, Brendan Hickey, Nasa Sinnott-Armstrong, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (H. Wang, C.A. Hogan, J.A. Miller, M.K. Sahoo, C. Huang, J. Zehnder, N. Sinnott-Armstrong, B.A. Pinsky); Stanford Health Care, Stanford (K.O. Mfuh, M. Sibai, B.A. Pinsky); Stanford University, Stanford (N. Sinnott-Armstrong)

Main Article

Figure 2

Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. Passing-Bablok regression and Bland-Altman plots for pools of 8 containing only 1 positive sample, tested by A and B) pooled LDT versus individual LDT (n = 23) (A, B); pooled Panther Fusion versus individual LDT (n = 25) (C, D); and pooled Panther Fusion versus pooled LDT (n = 32) (E, F). For the Passing-Bablok regression plots (A, C, and E), the solid line indicates the line of regression. 95% CIs are shaded in gray. The dashed line indicates the line of identity. The slope and intercept of the regression line are reported with 95% CIs in parentheses. For the Bland-Altman plots (B, D, and F), the solid line represents the mean difference in Ct value. 95% limits of agreement are shaded in gray. Panther Fusion is from Hologic (https://www.hologic.com). Ct, cycle threshold; LDT, laboratory-developed test; LOA, limits of agreement.

Figure 2. Performance of nucleic acid amplification tests for detection of severe acute respiratory syndrome coronavirus 2 in prospectively pooled specimens. Passing-Bablok regression and Bland-Altman plots for pools of 8 containing only 1 positive sample, tested by A and B) pooled LDT versus individual LDT (n = 23) (A, B); pooled Panther Fusion versus individual LDT (n = 25) (C, D); and pooled Panther Fusion versus pooled LDT (n = 32) (E, F). For the Passing-Bablok regression plots (A, C, and E), the solid line indicates the line of regression. 95% CIs are shaded in gray. The dashed line indicates the line of identity. The slope and intercept of the regression line are reported with 95% CIs in parentheses. For the Bland-Altman plots (B, D, and F), the solid line represents the mean difference in Ct value. 95% limits of agreement are shaded in gray. Panther Fusion is from Hologic (https://www.hologic.com). Ct, cycle threshold; LDT, laboratory-developed test; LOA, limits of agreement.

Main Article

Page created: September 29, 2020
Page updated: December 21, 2020
Page reviewed: December 21, 2020
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