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Volume 27, Number 1—January 2021
Research

Performance of Nucleic Acid Amplification Tests for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 in Prospectively Pooled Specimens

Hannah Wang, Catherine A. Hogan, Jacob A. Miller, Malaya K. Sahoo, ChunHong Huang, Kenji O. Mfuh, Mamdouh Sibai, James Zehnder, Brendan Hickey, Nasa Sinnott-Armstrong, and Benjamin A. PinskyComments to Author 
Author affiliations: Stanford University School of Medicine, Stanford, California, USA (H. Wang, C.A. Hogan, J.A. Miller, M.K. Sahoo, C. Huang, J. Zehnder, N. Sinnott-Armstrong, B.A. Pinsky); Stanford Health Care, Stanford (K.O. Mfuh, M. Sibai, B.A. Pinsky); Stanford University, Stanford (N. Sinnott-Armstrong)

Main Article

Table 3

Results of 8-sample pooled testing, by testing platform and number of positive specimens per pool (n = 110) for detection of severe acute respiratory syndrome coronavirus 2*

Pool no.
 Pooled testing
 Individual testing
 Total no. pools
LDT
 Panther Fusion
 Panther Aptima-M
 Panther Aptima-350
 Positive, 
(no. 1 PP, no. >1 PP)
 Negative
1 + + + + 30 (21, 9) 0 30
2 + + – + 2 (1, 1) 0 2
3 + + – – 0 (0, 0) 0 0
4 + – + + 0 (0, 0) 0 0
5 + – – + 0 (0, 0) 0 0
6 + – – – 1 (1, 0) 1† 2
7 – + + + 2 (2, 0) 0 2
8 – + – + 1 (1, 0) 0 1
9 – + – – 0 (0, 0) 1‡ 1
10 – – + + 2 (2, 0) 0 2
11 – – – + 1 (1, 0) 1§ 2
12 – – – – 7 (7, 0) 61 68
No. positive pools 34 36 34 39 46 (36, 10) – –
No. negative pools 76 74 76 71 – 64 –
Total no. pools 110 110 110 110 – – 110

*Panther Aptima with manufacturer-set relative light unit cutoff. Panther Aptima-350, Panther Aptima with relative light unit cutoff value >350 was considered positive; Ct, cycle threshold; LDT, laboratory-developed test; Pos, positive; RLU, relative light unit; 1 PP, 1 positive specimen in pool; >1 PP, >2 positive specimens in pool; –, negative; +, positive.

†False-positive LDT Ct value was 37.5.

‡False-positive Panther Fusion Ct value was 38.8.

§False-positive Panther Aptima-350 RLU value was 434.

Main Article

Page created: September 29, 2020
Page updated: December 21, 2020
Page reviewed: December 21, 2020
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