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Volume 27, Number 10—October 2021
Dispatch

Therapeutic Efficacy of Human Monoclonal Antibodies against Andes Virus Infection in Syrian Hamsters

Brandi N. Williamson1, Joseph Prescott1, Jose L. Garrido, Raymond A. Alvarez, Heinz Feldmann, and Maria I. BarríaComments to Author 
Author affiliations: National Institutes of Health, National Institute of Allergy and Infectious Diseases, Hamilton, Montana, USA (B.N. Williamson, J. Prescott, H. Feldmann); Robert Koch Institute, Berlin, Germany (J. Prescott); Ichor Biologics LLC, New York, New York, USA (J.L. Garrido, R.A. Alvarez); Universidad de Concepción, Concepción, Chile (J.L. Garrido, M.I. Barría); Universidad San Sebastián, Puerto Montt, Chile (M.I. Barría)

Main Article

Figure 1

In vivo efficacy of a cocktail of human mAbs specific for ANDV glycoprotein administered at days 5 and 9 postinfection in the Syrian hamster model of hantavirus cardiopulmonary syndrome. A) Syrian hamsters were inoculated intranasally with 200 FFU of ANDV and then administered intraperitoneally a cocktail of mAb (JL16 + MIB22, 25 mg/kg each) or isotype control (50 mg/kg) on day 5 and day 9 postinfection. B) Percentage of weight change monitored until 18 days postinfection, represented as the average per group. Error bars indicate 95% CIs. C) Statistical evaluation of survival by group. Survival was evaluated at p<0.0001 by Mantel-Cox log-rank test using GraphPad Prism (GraphPad Software, Inc., https://www.graphpad.com); p<0.05 was significant. d5 tx and d9 tx indicate treatment schedule (5 and 9 days postinfection). D) ANDV RNA copies per milligram of lung tissue on day 10 postinfection (d10) and 42 days postinfection (d42). Samples were compared to a standard curve using an in vitro transcribed ANDV RNA fragment of known small segment copy number. p values by unpaired t-test using GraphPad Prism. Symbols indicate geometric means; horizontal line indicates median; error bars indicate 95% CIs. ANDV, Andes virus; FFU, focus-forming units; mAbs, monoclonal antibodies.

Figure 1. In vivo efficacy of a cocktail of human mAbs specific for ANDV glycoprotein administered at days 5 and 9 postinfection in the Syrian hamster model of hantavirus cardiopulmonary syndrome. A) Syrian hamsters were inoculated intranasally with 200 FFU of ANDV and then administered intraperitoneally a cocktail of mAb (JL16 + MIB22, 25 mg/kg each) or isotype control (50 mg/kg) on day 5 and day 9 postinfection. B) Percentage of weight change monitored until 18 days postinfection, represented as the average per group. Error bars indicate 95% CIs. C) Statistical evaluation of survival by group. Survival was evaluated at p<0.0001 by Mantel-Cox log-rank test using GraphPad Prism (GraphPad Software, Inc., https://www.graphpad.com); p<0.05 was significant. d5 tx and d9 tx indicate treatment schedule (5 and 9 days postinfection). D) ANDV RNA copies per milligram of lung tissue on day 10 postinfection (d10) and 42 days postinfection (d42). Samples were compared to a standard curve using an in vitro transcribed ANDV RNA fragment of known small segment copy number. p values by unpaired t-test using GraphPad Prism. Symbols indicate geometric means; horizontal line indicates median; error bars indicate 95% CIs. ANDV, Andes virus; FFU, focus-forming units; mAbs, monoclonal antibodies.

Main Article

1These first authors contributed equally to this article.

Page created: August 31, 2021
Page updated: September 19, 2021
Page reviewed: September 19, 2021
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