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Volume 27, Number 11—November 2021
Research

Multidrug-Resistant Methicillin-Resistant Staphylococcus aureus Associated with Bacteremia and Monocyte Evasion, Rio de Janeiro, Brazil

Alice Slotfeldt Viana1, Ana Maria Nunes Botelho12, Ahmed M. Moustafa1, Craig L.K. Boge, Adriana Lucia Pires Ferreira, Maria Cícera da Silva Carvalho, Márcia Aparecida Guimarães, Bruno de Souza Scramignon Costa3, Marcos Corrêa de Mattos, Sabrina Pires Maciel, Juliana Echevarria-Lima, Apurva Narechania, Kelsey O’Brien, Chanelle Ryan, Jeffrey S. Gerber, Bernadete Teixeira Ferreira Carvalho, Agnes Marie Sá Figueiredo4, and Paul J. Planet4Comments to Author 
Author affiliations: Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil (A.S. Viana, A.M.N. Botelho, A.L.P. Ferreira, M.C.S. Carvalho, M.A. Guimarães, B.S.S. Costa, M.C. Mattos, S.P. Maciel, J. Echevarria-Lima, B.T.F. Carvalho, A.M.S. Figueiredo); Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania, USA (A.M. Moustafa, C.L.K. Boge, K. O’Brien, C. Ryan, J.S. Gerber, P.J. Planet); Diagnósticos da América S.A., Duque de Caxias, Brazil (A.L.P. Ferreira); American Museum of Natural History, New York, New York, USA (A. Narechania, P.J. Planet); University of Pennsylvania, Philadelphia (J.S. Gerber, P.J. Planet)

Main Article

Figure 6

Scatter plots showing evasion of phagocytosis by MRSA isolates, Rio de Janeiro, Brazil, 2014–2017. A) Phagocytosis rates for representatives of the phylogenetic groups CC5-Basal, CC5-IIA, and CC5-IIB. Four independent experiments were conducted for each lineage using 1 fluorescence-activated single cell sorting determination for each experiment. Horizontal lines indicate means; whiskers indicate SDs. B) Viable count of unphagocytosed (free) bacteria after 30-min interaction with THP-1 monocytes. Six independent experiments were considered, with 2 replicates per lineage. Horizontal lines indicate means; whiskers indicate SDs. Statistical analyses conducted using 1-way analysis of variance and Tukey multiple comparison test. CC, clonal complex; NS, not significant; MRSA, methicillin-resistant Staphylococcus aureus; SCC, staphylococcal cassette chromosome; ST, sequence type.

Figure 6. Scatter plots showing evasion of phagocytosis by MRSA isolates, Rio de Janeiro, Brazil, 2014–2017. A) Phagocytosis rates for representatives of the phylogenetic groups CC5-Basal, CC5-IIA, and CC5-IIB. Four independent experiments were conducted for each lineage using 1 fluorescence-activated single cell sorting determination for each experiment. Horizontal lines indicate means; whiskers indicate SDs. B) Viable count of unphagocytosed (free) bacteria after 30-min interaction with THP-1 monocytes. Six independent experiments were considered, with 2 replicates per lineage. Horizontal lines indicate means; whiskers indicate SDs. Statistical analyses conducted using 1-way analysis of variance and Tukey multiple comparison test. CC, clonal complex; NS, not significant; MRSA, methicillin-resistant Staphylococcus aureus; SCC, staphylococcal cassette chromosome; ST, sequence type.

Main Article

1These first authors contributed equally to this article.

2Current affiliation: Fluminense Federal University, Niteroi, Brazil.

3Current affiliation: Fundação Oswaldo Cruz, Rio de Janeiro, Brazil.

4These authors were co–principal investigators.

Page created: August 16, 2021
Page updated: October 19, 2021
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