Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 27, Number 12—December 2021
Dispatch

Experimental Oronasal Transmission of Chronic Wasting Disease Agent from White-Tailed Deer to Suffolk Sheep

Eric D. Cassmann, S. Jo Moore, and Justin J. GreenleeComments to Author 
Author affiliation: US Department of Agriculture, Ames, Iowa, USA

Main Article

Figure 2

Western blot analysis showing proteinase K‒resistant misfolded form of the prion protein (PrPSc) in brains of 4 Tg12 mice. Mice were intracranially inoculated with a homogenate made from retropharyngeal lymph node of a sheep oronasally inoculated with the agent of chronic wasting disease. Tg12 brain was prepared as a 10% (wt/vol) homogenate with phosphate-buffered saline. A total of 1 mg of tissue equivalent was treated with proteinase K (90 μg/mL) before electrophoresis. Immunodetection of PrPSc was performed overnight at 4°C with monoclonal antibody Sha31 (dilution 1:10,000). Left lane, molecular mass ladder. kDa, kilodaltons.

Figure 2. Western blot analysis showing proteinase K‒resistant misfolded form of the prion protein (PrPSc) in brains of 4 Tg12 mice. Mice were intracranially inoculated with a homogenate made from retropharyngeal lymph node of a sheep oronasally inoculated with the agent of chronic wasting disease. Tg12 brain was prepared as a 10% (wt/vol) homogenate with phosphate-buffered saline. A total of 1 mg of tissue equivalent was treated with proteinase K (90 μg/mL) before electrophoresis. Immunodetection of PrPSc was performed overnight at 4°C with monoclonal antibody Sha31 (dilution 1:10,000). Left lane, molecular mass ladder. kDa, kilodaltons.

Main Article

Page created: August 24, 2021
Page updated: November 19, 2021
Page reviewed: November 19, 2021
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external